INITIATION POINTS FOR DNA-REPLICATION IN NON-TRANSFORMED AND SIMIAN-VIRUS 40-TRANSFORMED CHINESE-HAMSTER LUNG-CELLS
INITIATION POINTS FOR DNA-REPLICATION IN NON-TRANSFORMED AND SIMIAN-VIRUS 40-TRANSFORMED CHINESE-HAMSTER LUNG-CELLS
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DOI：
10.1016/0092-8674(77)90297-5
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
OPPENHEIM, A
中科院分区：
文献类型：
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作者：
MARTIN, RG;OPPENHEIM, A
随机生长的中国仓鼠肺细胞用 3H-胸苷进行脉冲标记，并通过放射自显影使单个 DNA 纤维的复制叉可视化。当在完全培养基中生长时，野生型 SV-40 转化细胞比非转化细胞每单位长度 DNA 具有更多的分叉。在异亮氨酸耗尽的培养基中，野生型 SV-40 转化细胞每单位长度的分叉数量少于那些继续 DNA 复制的少数非转化细胞（占群体的 1-3%）。 SV-40 tsA 突变体转化的细胞在允许的温度下生长时，与未转化的细胞相比，在完全培养基中每单位长度具有更多的分叉，而在耗尽培养基中每单位长度具有更少的分叉。当在限制温度下生长时，tsA 转化细胞的行为与未转化细胞相似。
Randomly growing Chinese hamster lung cells were pulse-labeled with 3H-thymidine, and the replicating forks of individual DNA fibers were visualized by autoradiography. When grown in complete medium, wild-type SV-40-transformed cells had more forks per unit length of DNA than nontransformed cells. In isoleucine-depleted medium, wild-type SV-40-transformed cells had fewer forks per unit length than those few nontransformed cells (1-3% of the population) which continued DNA replication. Cells transformed by a tsA mutant of SV-40 when grown at the permissive temperature had more forks per unit length in complete medium and fewer forks per unit length in depleted medium than nontransformed cells. When grown at the restrictive temperature, the tsA-transformed cells behaved like nontransformed cells.