Cyclin Cln3p links G1 progression to hyphal and pseudohyphal development in Candida albicans
Cyclin Cln3p links G1 progression to hyphal and pseudohyphal development in Candida albicans
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DOI：
10.1128/ec.4.1.95-102.2005
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Whiteway, M
中科院分区：
文献类型：
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作者：
Bachewich, C;Whiteway, M
G（1）细胞周期蛋白在许多生物体中协调环境条件与生长和分化。在病原体白色念珠菌中，菌丝的分化是由环境因素诱导的，但不依赖于细胞周期。有趣的是，在酵母生长条件下抑制G（1）细胞周期蛋白Cln3p导致酵母细胞停滞在G（1），大小增加，然后发育成菌丝和假菌丝，随后恢复细胞周期。分化依赖于Efg1p，Cph1p和Ras1p，但Ras1p的缺失也是CLN3抑制的合成致死性的。相反，在环境诱导的菌丝中抑制CLN3并不抑制生长或细胞周期，这表明酵母和菌丝的细胞周期可能受到不同的调节。因此，G（1）细胞周期蛋白的缺失可以激活白色念珠菌的发育途径，并使其与正常环境对照的分化脱钩。这些数据表明，细胞周期的G（1）期可能在调节菌丝和假菌丝的发育中起关键作用。白色念珠菌。
G(1) cyclins coordinate environmental conditions with growth and differentiation in many organisms. In the pathogen Candida albicans, differentiation of hyphae is induced by environmental cues but in a cell cycle-independent manner. Intriguingly, repressing the G(1) cyclin Cln3p under yeast growth conditions caused yeast cells to arrest in G(1), increase in size, and then develop into hyphae and pseudohyphae, which subsequently resumed the cell cycle. Differentiation was dependent on Efg1p, Cph1p, and Ras1p, but absence of Ras1p was also synthetically lethal with repression of CLN3. In contrast, repressing CLN3 in environment-induced hyphae did not inhibit growth or the cell cycle, suggesting that yeast and hyphal cell cycles may be regulated differently. Therefore, absence of a G(1) cyclin can activate developmental pathways in C albicans and uncouple differentiation from the normal environmental controls. The data suggest that the G(1) phase of the cell cycle may therefore play a critical role in regulating hyphal and pseudohyphal development in C. albicans.