[Gerbils, As Experimental Animals (Meriones unguiculatus): Is A Good Role Model for Leishmania major?].
[Gerbils, As Experimental Animals (Meriones unguiculatus): Is A Good Role Model for Leishmania major?].
复制标题
[沙鼠，作为实验动物（Meriones unguiculatus）：是利什曼原虫的良好榜样吗？]。
DOI：
10.5152/tpd.2015.4300
复制
发表时间：
2015
影响因子：
--
通讯作者：
A. Özbilgin
中科院分区：
文献类型：
--
作者：
S. Bakırcı;H. Bilgic;Onur Köse;Ayça Aksulu;Selin Hacılarlıoğlu;T. Karagenç;İbrahim Çavuş;A. Özbilgin
目的
本研究旨在观察L.采用经典的涂片技术，接种到富集的Novy-MacNeal-Nicolle（NNN）培养物中，并通过聚合酶链反应（PCR）检测，对长爪沙鼠（Meriones unguiculatus）的主要无鞭毛体进行感染诊断。
方法
在本研究中，L.一名18岁的男子主要隔离者，居住在土耳其的Bitlis省。该菌株也用于感染沙鼠。10只沙鼠共接种1 × 10（8）个/mL前鞭毛体。对感染的沙鼠进行尸检，以监测寄生虫的内脏化趋势。从每只动物中制备组织样品，通过Giemsa染色并接种到NNN培养物中。然而，进行实时PCR测定以确认临床材料的感染。
结果
Giemsa染色的组织涂片的检查显示，感染硕大利什曼原虫的动物在感染后第一个月在所有组织中对利什曼原虫无鞭毛体呈阳性，并且利什曼原虫前鞭毛体在26°C下在含有NNN的培养瓶中培养。核糖体内部转录间隔区1（ITS-1）PCR解链曲线分析显示与L.少校
结论
结果表明，本研究证实了姬姆萨染色涂片和PCR，内脏化和传播的L。主要无鞭毛体，沙鼠人畜共患皮肤利什曼病的主要原因。
OBJECTIVE
This study aimed to observation the possible visceralization tendency and dissemination of L. major amastigotes in gerbils (Meriones unguiculatus) using a classic smear technique, inoculated into enriched Novy-MacNeal-Nicolle (NNN) culture and polymerase chain reaction (PCR) assay for diagnosis of infection.
METHODS
In this study, L. major isolated from a man who 18 years old, living in Bitlis province of Turkey. This strain also was utilized to infect gerbils. A total of 1 × 10(8)/mL promastigotes were inoculated to 10 gerbils. Necropsy was performed on infected gerbils for monitoring the visceralization tendency of the parasites. Tissue samples were prepared from each animal and stained by Giemsa and inoculated into NNN culture. However, a real-time PCR assay was performed to confirm the infection the clinical material.
RESULTS
Examination of Giemsa-stained tissue smears showed that infected animals with L.major were positive for Leishmania amastigotes in all tissues at the first month post infection and Leishmania promastigotes were cultured at 26°C in culture flasks containing NNN. Melting curve analyses of ribozomal internal transcribed spacer 1 (ITS-1) PCR showed the peak concordant with L. major.
CONCLUSION
As a result, the present study confirmed by both Giemsa-stained smears and PCR, visceralization and dissemination of L. major amastigotes, the principal cause of zoonotic cutaneous leishmaniasis in gerbils.