Immunohistochemical Detection of Urokinase Plasminogen Activator and Urokinase Plasminogen Activator Receptor in Canine Vascular Endothelial Tumours
Immunohistochemical Detection of Urokinase Plasminogen Activator and Urokinase Plasminogen Activator Receptor in Canine Vascular Endothelial Tumours
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DOI：
10.1016/j.jcpa.2015.07.003
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发表时间：
2015-11-01
影响因子：
0.8
通讯作者：
Sakai, H.
中科院分区：
文献类型：
--
作者：
Anwar, Sh.;Yanai, T.;Sakai, H.
应用免疫组织化学方法检测了57例犬原发性血管瘤（HSAs）、26例犬皮肤血管瘤（HAs）及对照犬皮肤肉芽组织中尿激酶型纤溶酶原激活物（uPA）及其受体（uPAR）的表达。在HSA和HA组织中估计uPA/uPAR表达与Ki 67标记指数（LI）之间的相关性。uPA在脾HSAs和非脾HSAs中的表达率分别为73.2%和75.0%。所有检测的HSA组织均表达uPAR。这两种分子在HSA中的表达显着高于皮肤HA（uPA为3.8%，uPAR为30.7%）。uPA（+）/uPAR（+）HSAs的平均Ki 67 LI显著高于uPA（-）/uPAR（+）HSAs和HA组织（平均值+/-SD分别为32.8 +/- 15.3、15.2 +/- 7.2和2.1 +/- 0.7; P < 0.05）。这些结果表明，uPA和uPAR在犬HSA的恶性增殖中起着重要的作用，无论肿瘤的原发来源。(C)2015爱思唯尔有限公司版权所有。
Immunohistochemistry was used to assess the expression of urokinase plasminogen activator (uPA) and uPA receptor (uPAR) in 57 canine primary haemangiosarcomas (HSAs), 26 canine cutaneous haemangiomas (HAs) and in control sections of canine cutaneous granulation tissue. The correlation between uPA/uPAR expression and the Ki67 labelling index (LI) was estimated in the HSA and HA tissues. uPA was expressed by 73.2% and 75.0% of splenic HSAs and non-splenic HSAs, respectively. All HSA tissues tested expressed uPAR. Expression of both molecules was significantly higher in HSAs than in cutaneous HAs (3.8% for uPA and 30.7% for uPAR). The average Ki67 LI of the uPA(+)/uPAR(+) HSAs was significantly higher than that of uPA(-)/uPAR(+) HSAs and HA tissues (mean +/- SDs 32.8 +/- 15.3, 15.2 +/- 7.2 and 2.1 +/- 0.7, respectively; P < 0.05). These results suggest that uPA and uPAR play a significant role in the malignant proliferation of canine HSA, regardless of the primary origin of the tumour. (C) 2015 Elsevier Ltd. All rights reserved.