Genomic structure of the whole D-J-C clusters and the upstream region coding V segments of the TRB locus in pig
Genomic structure of the whole D-J-C clusters and the upstream region coding V segments of the TRB locus in pig
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DOI：
10.1016/j.dci.2009.06.006
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发表时间：
2009-10-01
影响因子：
2.9
通讯作者：
Uenishi, Hirohide
中科院分区：
文献类型：
--
作者：
Eguchi-Ogawa, Tomoko;Toki, Daisuke;Uenishi, Hirohide
在脊椎动物免疫系统中，T细胞在宿主防御微生物或病毒感染中发挥核心作用。以前的研究表明，至少有两套TRBD-J-C簇在猪基因组中。在这项研究中，我们确定了212，193 bp的连续猪基因组序列覆盖整个TRBC区域。在TRBD-J-C簇的附近，EPHB 6、TRPV 6、TRY和10个TRBV基因是保守的。有趣的是，在该序列中鉴定出三个TRBD-J-C簇;每个TRBD-J-C簇由一个TRBD和七个TRBJ片段组成，其中一个TRBC区域由四个外显子组成。重复序列的分布和系统发育分析表明，TRBD-J-C簇位于3个簇的中心，具有与其它簇结合的结构。大多数TRBJ片段可在公共数据库中获得，这表明所有三个TRBD-J-C簇在猪中都有功能。(C)2009爱思唯尔有限公司保留所有权利。
In the vertebrate immune system, T cells play a central role in host defense against microbial or viral infection. Previous studies suggested that at least two sets of TRBD-J-C clusters are harbored in the porcine genome. In this study, we determined 212,193 bp of a continuous porcine genomic sequence covering the entire TRBC region. EPHB6, TRPV6, TRY, and ten TRBV genes were conserved in the vicinity of the TRBD-J-C clusters. Interestingly, three TRBD-J-C clusters were identified in this sequence; each TRBD-J-C cluster consisted of one TRBD and seven TRBJ segments, with one TRBC region composed of four exons. The distribution of repetitive sequences and phylogenetic analysis indicated that the TRBD-J-C cluster, located at the center of the three clusters identified, had a structure combined with the others. Most of the TRBJ segments were available in public databases, suggesting that all three TRBD-J-C clusters are functional in pigs. (C) 2009 Elsevier Ltd. All rights reserved.