Exploration of the Neisseria Resistome Reveals Resistance Mechanisms in Commensals That May Be Acquired by N. gonorrhoeae through Horizontal Gene Transfer.
Exploration of the Neisseria Resistome Reveals Resistance Mechanisms in Commensals That May Be Acquired by N. gonorrhoeae through Horizontal Gene Transfer.
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奈瑟氏球菌耐药基因组的研究揭示了可能被奈瑟氏球菌获得的共生体的耐药机制。淋病通过水平基因转移。
DOI：
10.3390/antibiotics9100656
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发表时间：
2020-09-30
期刊：
影响因子：
--
通讯作者：
Wadsworth CB
中科院分区：
文献类型：
--
作者：
Fiore MA;Raisman JC;Wong NH;Hudson AO;Wadsworth CB
非致病性奈瑟菌转移突变编码抗生素耐药性，其致病性相关淋球菌。然而，耐药基因型和随后的表型的属内的非病原体已经很少被描述。在这里，我们的特点的最低抑菌浓度（MIC）的一组奈瑟氏球菌（n = 26）-包括几个细菌物种-一套不同的抗生素。我们还使用全基因组测序和综合抗生素耐药数据库耐药基因标识符（RGI）平台来预测推定的耐药编码突变。对青霉素（n = 5/26）、头孢曲松（n = 2/26）、头孢克肟（n = 3/26）、四环素（n = 10/26）、阿奇霉素（n = 11/26）和环丙沙星（n = 4/26）等所有抗菌药物均产生耐药。几种突变的存在与MIC增加明确相关，如DNA促旋酶亚基A（gyrA）（S91 F）和环丙沙星，四环素耐药蛋白（tetM）和30 S核糖体蛋白S10（rpsJ）（V57 M）和四环素，以及TEM型β-内酰胺酶和青霉素。然而，突变与大环内酯类和头孢菌素耐药性强相关性尚不确定。这项工作是对非致病性奈瑟菌所携带的耐药编码突变的初步探索，这将最终有助于对可能被奈瑟菌快速获得的新型耐药机制进行前瞻性监测。淋病
Nonpathogenic Neisseria transfer mutations encoding antibiotic resistance to their pathogenic relative Neisseria gonorrhoeae. However, the resistance genotypes and subsequent phenotypes of nonpathogens within the genus have been described infrequently. Here, we characterize the minimum inhibitory concentrations (MICs) of a panel of Neisseria (n = 26)—including several commensal species—to a suite of diverse antibiotics. We furthermore use whole genome sequencing and the Comprehensive Antibiotic Resistance Database Resistance Gene Identifier (RGI) platform to predict putative resistance-encoding mutations. Resistant isolates to all tested antimicrobials including penicillin (n = 5/26), ceftriaxone (n = 2/26), cefixime (n = 3/26), tetracycline (n = 10/26), azithromycin (n = 11/26), and ciprofloxacin (n = 4/26) were found. In total, 63 distinct mutations were predicted by RGI to be involved in resistance. The presence of several mutations had clear associations with increased MIC such as DNA gyrase subunit A (gyrA) (S91F) and ciprofloxacin, tetracycline resistance protein (tetM) and 30S ribosomal protein S10 (rpsJ) (V57M) and tetracycline, and TEM-type β-lactamases and penicillin. However, mutations with strong associations to macrolide and cephalosporin resistance were not conclusive. This work serves as an initial exploration into the resistance-encoding mutations harbored by nonpathogenic Neisseria, which will ultimately aid in prospective surveillance for novel resistance mechanisms that may be rapidly acquired by N. gonorrhoeae.
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影响因子：
4.9
作者：
Hu, M;Nandi, S;Nicholas, RA
通讯作者：
Nicholas, RA
影响因子：
9.4
作者：
Demczuk, Walter;Martin, Irene;Mulvey, Michael R.
通讯作者：
Mulvey, Michael R.
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
DOI：
10.1126/science.1220761
发表时间：
2012-08-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Forsberg KJ;Reyes A;Wang B;Selleck EM;Sommer MO;Dantas G
通讯作者：
Dantas G
影响因子：
4.9
作者：
Gill, MJ;Simjee, S;Ison, CA
通讯作者：
Ison, CA