Self-aggregation inhibits the photonuclease activity of porphyrins.
Self-aggregation inhibits the photonuclease activity of porphyrins.
复制标题
自聚集抑制卟啉的光核酸酶活性。
DOI：
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发表时间：
2003
影响因子：
1.7
通讯作者：
T. Uno
中科院分区：
文献类型：
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作者：
Katsumasa Aoki;Y. Ishikawa;Miyuki Oyama;Y. Tomisugi;T. Uno
合成了一系列具有4个氨基烷基的卟啉衍生物，并利用吸收光谱和圆二色光谱研究了它们与DNA的结合性能。氨丙基链被发现，以促进结合，和bisignate诱导的CD光谱显示，卟啉是自堆叠的DNA表面。同时对卟啉的光核酸酶活性进行了研究，发现氨丙基卟啉的光核酸酶活性最高。活性随卟啉负载量的增加而增加，但较高的负载量导致活性降低。该抑制步骤对应于卟啉的聚集。因此，认为聚集是为了将内部卟啉与溶剂屏蔽，抑制活性氧物种的产生。
A series of tentacle porphyrins having four aminoalkyl groups at the periphery was synthesized, and the DNA binding properties were investigated by absorption and circular dichroism (CD) spectroscopic methods. The aminopropyl chain was found to facilitate binding, and bisignate induced CD spectra revealed that the porphyrins are self-stacked on the DNA surface. The photonuclease activity of the tentacle porphyrins was also studied, and the aminopropylporphyrin showed the highest activity. The activity increased in proportion to the porphyrin load, but higher loads resulted in the decrease of activity. This inhibitory step corresponded to aggregation of the porphyrin. Thus, the aggregation was suggested to shield the inner porphyrin from the solvent, the production of active oxygen species being suppressed.