Annulate lamellae: comparison of antigenic epitopes of annulate lamellae membranes with the nuclear envelope.
Annulate lamellae: comparison of antigenic epitopes of annulate lamellae membranes with the nuclear envelope.
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DOI：
10.1083/jcb.107.4.1299
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发表时间：
1988-10
期刊：
影响因子：
--
通讯作者：
Merisko EM
中科院分区：
文献类型：
--
作者：
Chen TY;Merisko EM
环状片层（Annulate lamellate，AL）是配子和肿瘤细胞分化过程中常见的一种膜性结构。尽管有许多形态学研究，但该膜系统的功能和生化组成还不清楚。在这项研究中，我们研究了长春碱处理小鼠L细胞的AL膜系统，用免疫细胞化学和Western印迹分析。我们的结果表明，针对核膜层蛋白的抗体，即，核纤层蛋白A、B和C不与AL膜系统的成分发生交叉反应。此外，AL特异性抗体未能与核膜反应，并且反应最低，仅产生超过其他细胞组分的背景染色。这些数据表明，AL膜系统有一个独特的分子组成，这是抗原不同的其他亚细胞结构。
Annulate lamellae (AL) are a membranous structure frequently observed in differentiating gametes and tumor cells. In spite of numerous morphological studies, the function and biochemical composition of this membrane system are not well understood. In this study, we have examined the AL membrane system of vinblastine-treated mouse L cells using immunocytochemistry and Western blot analysis. Our results show that antibodies directed against nuclear envelope lamins, i.e., lamins A, B, and C, did not cross react with constituents of the AL membrane system. Furthermore an AL-specific antibody failed to react with the nuclear envelope and reacted minimally producing only a background stain over other cellular components. The data suggest that the AL membrane system has a distinct molecular make-up that is antigenically distinct from that of other subcellular structures.