Hypothalamic prepro-orexin mRNA level is inversely correlated to the non-rapid eye movement sleep level in high-fat diet-induced obese mice
Hypothalamic prepro-orexin mRNA level is inversely correlated to the non-rapid eye movement sleep level in high-fat diet-induced obese mice
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DOI：
10.1016/j.orcp.2013.01.005
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发表时间：
2013-07-01
影响因子：
4.3
通讯作者：
Kimura, Kazuhiro
中科院分区：
文献类型：
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作者：
Tanno, Shogo;Terao, Akira;Kimura, Kazuhiro
食欲素是下丘脑的一种神经肽，在调节和维持睡眠/觉醒状态和能量平衡中起重要作用。为了评估食欲素系统的改变是否与肥胖症中观察到的睡眠/觉醒异常相关，我们检测了正常饮食（ND）和高脂饮食（HFD）诱导的肥胖小鼠下丘脑中前食欲素原、食欲素受体1型（食欲素1 r）和食欲素受体2型（oxexin 2 r）的mRNA表达。我们还比较了它们与睡眠/觉醒的关系。将24只4周龄雄性C57 BL/6 J小鼠随机分为三组，接受以下治疗：（1）ND 17周;（2）HFD 17周;和（3）ND 7周和HFD另外10周。喂食HFD 10-17周的小鼠体重为ND组平均体重的112-150%。在HFD喂养的小鼠中，每日非快速眼动（NREM）睡眠量显著增加。这些变化伴随着每次NREM睡眠发作次数的增加和持续时间的减少。此外，短暂的觉醒（
Orexins are hypothalamic neuropeptides, which play important roles in the regulation and maintenance of sleep/wakefulness states and energy homeostasis. To evaluate whether alterations in orexin system is associated with the sleep/wakefulness abnormalities observed in obesity, we examined the mRNA expression of prepro-orexin, orexin receptor type 1 (orexin 1r), and orexin receptor type 2 (oxexin 2r) in the hypothalamus in mice fed with a normal diet (ND) and high-fat diet (HFD)-induced obese mice. We also compared their relationships with sleep/wakefulness. Twenty-four, 4-week-old, male C57BL/6J mice were divided randomly into three groups, which received the following: (1) ND for 17 weeks; (2) HFD for 17 weeks; and (3) ND for 7 weeks and HFD for a further 10 weeks. The body weights of mice fed the HFD for 10-17 weeks were 112-150% of the average body weight of the ND group. The daily amount of non-rapid eye movement (NREM) sleep increased significantly in HFD-fed mice. These changes were accompanied by increases in the number but decreases in the duration of each NREM sleep episode. In addition, brief awakenings (