Telomerase Reverse Transcriptase Expression in Mouse Endometrium During Reepithelialization and Regeneration in a Menses-Like Model
Telomerase Reverse Transcriptase Expression in Mouse Endometrium During Reepithelialization and Regeneration in a Menses-Like Model
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DOI：
10.1089/scd.2018.0133
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发表时间：
2018-11-27
影响因子：
4
通讯作者：
Gargett, Caroline E.
中科院分区：
文献类型：
--
作者：
Cousins, Fiona L.;Dorien, F. O.;Gargett, Caroline E.
子宫内膜的再生能力归因于驻留的干细胞/祖细胞。已有许多人子宫内膜干/祖细胞的干/祖细胞标记物的报道；然而，在小鼠身上缺乏方便的标记物使子宫内膜再生的实验研究变得困难。我们最近鉴定了子宫内膜上皮细胞、内皮细胞和免疫细胞，它们表达干/祖细胞标记物--小鼠端粒酶逆转录酶(MTert)。在这项研究中，我们研究了mTert启动子活性的绿色荧光蛋白(GFP)报告(mTert-GFP)在月经样事件后子宫内膜再生中的表达模式。MTert-GFP的表达标志着T细胞和成熟巨噬细胞的上皮下亚群，并可能在免疫细胞调节的修复中发挥作用。在再上皮化区附近发现了mTert-GFP阳性的上皮细胞群，这可能突出了mTert在子宫内膜上皮的修复和再生中的作用。
The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration difficult. We recently identified endometrial epithelial, endothelial, and immune cells, which express a reporter for the stem/progenitor marker, mouse telomerase reverse transcriptase (mTert). In this study, we investigate the expression pattern of a green fluorescent protein (GFP) reporter for mTert promoter activity (mTert-GFP) in endometrial regeneration following a menses-like event. mTert-GFP expression marks subepithelial populations of T cells and mature macrophages and may play a role in immune cell regulated repair. Clusters of mTert-GFP-positive epithelial cells were identified close to areas of reepithelialization and possibly highlight a role for mTert in the repair and regeneration of the endometrial epithelium.