Isolation and characterization of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae and Klebsiella variicola strains from various environments
Isolation and characterization of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae and Klebsiella variicola strains from various environments
复制标题
不同环境中异养硝化和好氧反硝化肺炎克雷伯菌和水痘克雷伯菌菌株的分离和鉴定
DOI：
10.1111/jam.13703
复制
发表时间：
2018-05-01
影响因子：
4
通讯作者：
Shu, Q. L.
中科院分区：
文献类型：
--
作者：
Feng, Y.;Feng, J.;Shu, Q. L.
目的：从不同自然环境中分离新型异养硝化和好氧反硝化克雷伯氏菌（Klebsiella pneumoniae）和变孢克雷伯氏菌（Klebsiella variicola），并研究其脱氮过程。pneumoniae和3株K.从粪便、污水、植物表面和污泥中成功地分离出天花菌。从新菌株中成功扩增出一个1674 bp的羟胺氧化酶基因片段。7种K. pneumoniae菌株的降解率分别为9642- 9738%、6127-8278%和100%，相应的降解率为482-649（高于以前报道的K. pneumoniae CF-S9和EGD-HP 19-C）分别为115-138和148- 333 mgl（-1）h（-1）。3种K.菌株的降解率分别为9501- 9615%、6860 - 7837%和100%，相应的降解率分别为479-95、114-174和167- 444 mgl（-1）h（-1）。酸克雷污泥中的菌株SD-3表现出最好的异养硝化和好氧反硝化能力。同时，对分离的菌株进行同步硝化反硝化试验，结果表明，K.肺炎克雷伯菌和肺炎克雷伯菌。Variicola（分离自一个新的自然环境）在地球氮循环中起着重要的作用。本研究的意义和影响本研究是少数成功分离出K. pneumoniae在各种自然环境中具有异养硝化和好氧反硝化能力。生理特性K. Variicola具有异养硝化和好氧硝化的能力是首次报道。此外，本研究可能为废水脱氮提供替代的微生物资源。
Aims We sought to isolate novel heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae and Klebsiella variicola strains from various natural environments and characterize their nitrogen removal processes.Methods and Results Ten novel Klebsiella strains with heterotrophic nitrification and aerobic denitrification abilities, including seven K. pneumoniae and three K. variicola, were successfully isolated from faeces, sewage, plant surfaces and sludge. A 1674-bp fragment of the hydroxylamine oxidase (hao) gene was successfully amplified from the novel strains. The removal rates of ammonium, nitrate and nitrite of the seven K. pneumoniae isolates were 9642-9738%, 6127-8278% and 100%, respectively, and the corresponding degradation ratios were 482-649 (higher than previously reported for K. pneumoniae CF-S9 and EGD-HP19-C), 115-138 and 148-333mgl(-1)h(-1), respectively. The removal rates of ammonium, nitrate and nitrite of the three K. variicola isolates were 9501-9615%,6860--7837% and 100%, respectively, and the corresponding degradation ratios were 479-95, 114-174 and 167-444mgl(-1)h(-1), respectively. The K. variicola strain sd-3 from sludge exhibited the best heterotrophic nitrification and aerobic denitrification abilities among the isolates. Meanwhile, the results of simultaneous nitrification and denitrification assays with all isolated strains showed that ammonium was removed prior to nitrate or nitrite.Conclusions Our results indicate that K. pneumoniae and K. variicola (isolated from a novel natural environment) play an important role in the Earth's nitrogen cycle in various natural environments.Significance and Impact of the Study This study is one of only a few works to successfully isolate K. pneumoniae with heterotrophic nitrification and aerobic denitrification abilities in various natural environments. The physiological characterization K. variicola as having abilities to heterotrophically nitrify and aerobically denitrify is the first to be reported. Moreover, this study may provide alternative microbial resources for the removal of nitrogen from wastewater.