The human cytomegalovirus 86-kilodalton major immediate-early protein interacts physically and functionally with histone acetyltransferase P/CAF
The human cytomegalovirus 86-kilodalton major immediate-early protein interacts physically and functionally with histone acetyltransferase P/CAF
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DOI：
10.1128/jvi.74.16.7230-7237.2000
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发表时间：
2000-08-01
影响因子：
5.4
通讯作者：
Sinclair, JH
中科院分区：
文献类型：
--
作者：
Bryant, LA;Mixon, P;Sinclair, JH
人巨细胞病毒（HCMV）的主要立即早期蛋白在生产性感染过程中控制病毒和细胞基因表达中起着关键作用。除了负性自动调节其自身的启动子，HCMV 86-kDa主要立即早期蛋白（IE86）激活病毒早期基因表达，并且已知是细胞基因的混杂转录调节因子。IEg 6似乎充当多模式转录因子。它能够直接结合靶启动子以激活转录，但也能够在上游结合因子如CREB/ATF和基础转录复合物之间桥接，以及直接与一般转录因子如TATA结合蛋白和TFIIB相互作用。我们现在表明，IE86也能够在感染过程中直接与组蛋白乙酰转移酶相互作用。这些因子中至少有一个是组蛋白乙酰转移酶CBP相关因子（P/CAF）。此外，我们表明，这种相互作用的结果在协同反式激活IE86的IE86响应启动子。通过IE86将这些染色质重塑因子募集到靶向启动子可能有助于解释这种病毒蛋白作为细胞基因的混杂反式激活因子的能力。
The major immediate-early proteins of human cytomegalovirus (HCMV) play a pivotal role in controlling viral and cellular gene expression during productive infection. As well as negatively autoregulating its own promoter, the HCMV 86-kDa major immediate early protein (IE86) activates viral early gene expression and is known to be a promiscuous transcriptional regulator of cellular genes. IEg6 appears to act as a multimodal transcription factor. It is able to bind directly to target promoters to activate transcription but is also able to bridge between upstream binding factors such as CREB/ATF and the basal transcription complex as well as interacting directly with general transcription factors such as TATA-binding protein and TFIIB. We now show that IE86 is also able to interact directly with histone acetyltransferases during infection. At Least one of these factors is the histone acetyltransferase CBP-associated factor (P/CAF). Furthermore, we show that this interaction results in synergistic transactivation by IE86 of IE86-responsive promoters. Recruitment of such chromatin-remodeling factors to target promoters by IE86 may help explain the ability of this viral protein to act as a promiscuous transactivator of cellular genes.