In situ determination of structure and fluctuations of coexisting fluid membrane domains.
In situ determination of structure and fluctuations of coexisting fluid membrane domains.
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DOI：
10.1016/j.bpj.2014.11.3488
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发表时间：
2015-02-17
影响因子：
3.4
通讯作者：
Pabst, Georg
中科院分区：
文献类型：
--
作者：
Heftberger, Peter;Kollmitzer, Benjamin;Rieder, Alexander A.;Amenitsch, Heinz;Pabst, Georg
膜域的生物物理理解需要准确的知识，其结构细节和弹性。我们报告了一个全球性的小角度X射线散射数据分析技术共存的液体有序（Lo）和液体无序（Ld）域在完全水合的多层囊泡。这使得他们能够详细分析膜厚度、每种脂质面积、烃链长度和弯曲波动的差异，如在不同胆固醇浓度下两种三元混合物（DOPC/DSPC/CHOL和DOPC/DPPC/CHOL）所示。Lo结构域被发现是100 μ m厚，横向高达20 μ 2/脂质更凝聚比Ld结构域。它们的弯曲波动也减少了0.65%。胆固醇浓度的增加引起了显着的结构特性的变化，而其对Lo性能的影响是微不足道的。我们进一步观察到，温度诱导的Lo域的熔化与胆固醇扩散到Ld域相关，并由Lo/Ld厚度差控制。
Biophysical understanding of membrane domains requires accurate knowledge of their structural details and elasticity. We report on a global small angle x-ray scattering data analysis technique for coexisting liquid-ordered (Lo) and liquid-disordered (Ld) domains in fully hydrated multilamellar vesicles. This enabled their detailed analysis for differences in membrane thickness, area per lipid, hydrocarbon chain length, and bending fluctuation as demonstrated for two ternary mixtures (DOPC/DSPC/CHOL and DOPC/DPPC/CHOL) at different cholesterol concentrations. Lo domains were found to be ∼10 Å thicker, and laterally up to 20 Å2/lipid more condensed than Ld domains. Their bending fluctuations were also reduced by ∼65%. Increase of cholesterol concentration caused significant changes in structural properties of Ld, while its influence on Lo properties was marginal. We further observed that temperature-induced melting of Lo domains is associated with a diffusion of cholesterol to Ld domains and controlled by Lo/Ld thickness differences.
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影响因子：
6.1
作者：
Heftberger P;Kollmitzer B;Heberle FA;Pan J;Rappolt M;Amenitsch H;Kučerka N;Katsaras J;Pabst G
通讯作者：
Pabst G
影响因子：
5.5
作者：
Khelashvili, George;Kollmitzer, Benjamin;Heftberger, Peter;Pabst, Georg;Harries, Daniel
通讯作者：
Harries, Daniel
影响因子：
3.4
作者：
Heberle, Frederick A.;Wu, Jing;Feigenson, Gerald W.
通讯作者：
Feigenson, Gerald W.
影响因子：
3.9
作者：
Buboltz, Jeffrey T.;Bwalya, Charles;Schutzer, Matthew
通讯作者：
Schutzer, Matthew
影响因子：
3.4
作者：
Chiang, YW;Shimoyama, Y;Freed, JH
通讯作者：
Freed, JH