Combined effects of ozone and nitrogen on secondary compounds, amino acids, and aphid performance in Scots pine
Combined effects of ozone and nitrogen on secondary compounds, amino acids, and aphid performance in Scots pine
复制标题
DOI：
10.2134/jeq2000.00472425002900010042x
复制
发表时间：
2000-01-01
影响因子：
2.4
通讯作者：
Holopainen, T
中科院分区：
文献类型：
--
作者：
Kainulainen, P;Holopainen, JK;Holopainen, T
在两个不同的生长箱试验中，研究了O-3和N素对樟子松L苗木的综合效应。在0、0.075、0.15和0.3亩的L/L溶液中，苗木在8h/d、5d/wk和5wk的时间内，分别施以低、中、高水平的氮肥。臭氧和氮素有效性对几种主要和次生代谢产物的浓度有影响，试验1(幼苗停止生长)代谢产物的变化比试验1大。2(苗木生长活跃)。总体而言，高O-3暴露水平显著降低了单萜的浓度，增加了树脂酸的浓度。总酚浓度不受O-3暴露的影响。中等氮肥水平下，单萜和树脂酸的浓度大多低于低氮水平和高氮水平，而总酚浓度随着氮素有效性的提高而降低。试验一，在O-3浓度为0.3亩L/L的条件下，游离氨基酸浓度显著升高，氮素供应对氨基酸浓度影响不显著。在实验中1、O-3和N对松突裂殖吸虫的MRGR均有显著影响。在实验中2、O-3和N对樟子松幼虫雌虫和若虫体重及繁殖总若虫数有显著影响，但交互作用很小，说明N对O-3诱导的樟子松苗期初、次生物质和麦长管蚜行为的影响不显著。
Combined effects of O-3 and N supply on Scots pine (Pinus sylvestris L,) were studied in two separate growth chamber experiments exposing seedlings to 0, 0.075, 0.15, and 0.3 mu L/L of O-3 during 8 h/d, 5 d/wk for a period of 5 wk, Seedlings were fertilized with low, medium, and high levels of N. Ozone and N availability affected concentrations of several primary and secondary metabolites, More changes on metabolites were detected in Exp, 1 (with seedlings ceasing their annual growth) than in Exp. 2 (with seedlings actively growing). Overall, high O-3 exposure levels significantly decreased concentrations of monoterpenes and increased concentrations of resin acids. Concentrations of total phenolics were not affected by O-3 exposure. Mostly lower concentrations of monoterpenes and resin acids were found at a medium N-fertilization level than at low and high N-fertilization levels, while total phenolic concentration decreased by enhanced N availability. In Exp, 1, significantly elevated concentrations of free amino acids were found at O-3 concentration of 0.3 mu L/L, Nitrogen availability did not have remarkable effects on amino acid concentrations. in Exp. 1, both O-3 and N had a significant effect on the MRGR of the aphid Schizolachnus pineti. In Exp. 2, the weight of the females and nymphs and the total number of reproduced nymphs were significantly affected by O-3 and N, Only a few interaction effects were found, suggesting that the N supply does not significantly modify O-3-induced effects on studied primary and secondary compounds and aphid performance in Scots pine seedlings.