Genomic representation of the Hind II 1.9 kb repeated DNA.
Genomic representation of the Hind II 1.9 kb repeated DNA.
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Hind II 1.9 kb 重复 DNA 的基因组表示。
DOI：
10.1093/nar/10.10.3221
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发表时间：
1982
影响因子：
14.9
通讯作者：
Biro,PA
中科院分区：
文献类型：
--
作者：
Manuelidis,L;Biro,PA
研究了与克隆的 Hind III 1.9 kb 重复 DNA 片段同源的序列的基因组表示和组织。大约 80% 的同源重复 DNA 包含在 1.9 kb 的基因组 Hind III 切割带中。双消化研究表明，大多数基因组家族遵循测序克隆的排列，具有与少数碱基变化兼容的较小限制性切割变化。绘制了 1.9 kb 序列外部的常见限制性位点，克隆序列片段的杂交表明 1.9 kb DNA 本身不是串联重复的。与序列同源的Kpn I条带包含特定的重复区域，并且这些较大片段的分子量可以简单地解释。常见外部限制性位点的图谱表明，在某些情况下（但不是所有情况），重复序列可以组织成≥ 5.5 kb 的较大定义块。在一些情况下，与重复相邻的侧翼区域可能包含常见的DNA元件，例如其他重复的DNA序列，或者可能是1.9kb序列的重排片段。这表明，尽管1.9 kb的序列不是严格连续的，但人类基因组中至少有一些重复序列以簇状或闰阵列的形式排列。 1.9 kb 序列的一个区域与小鼠重复 DNA 杂交，表明其同源性超出了灵长类动物。
The genomic representation and organization of sequences homologous to a cloned Hind III 1.9 kb repeated DNA fragment were studied. Approximately 80% of homologous repeated DNA was contained in a genomic Hind III cleavage band of 1.9 kb. Double digestion studies indicated that the genomic family, in the majority, followed the arrangement of the sequenced clone, with minor restriction cleavage variations compatible with a few base changes. Common restriction sites external to the 1.9 kb sequence were mapped, and hybridization of segments of the cloned sequence indicated the 1.9 kb DNA was itself not tandemly repeated. Kpn I bands which were homologous to the sequence contained specific regions of the repeat, and the molecular weight of these larger fragments could be simply explained. Mapping of common external restriction sites indicated that in some but not all cases the repeat could be organized in larger defined blocks of ≥ 5.5 kb. In some instances, flanking regions adjacent to the repeat may contain common DNA elements such as other repeated DNA sequences, or possibly rearranged segments of the 1.9 kb sequence. It is suggested that although the 1.9 kb sequence is not strictly contiguous, at least some of these repeated sequences in the human genome are arranged in clustered or intercalary arrays. A region of the 1.9 kb sequence hybidized to a mouse repeated DNA, indicating homology beyond the primates.