A new near infrared photosensitizing nanoplatform containing blue-emitting up-conversion nanoparticles and hypocrellin A for photodynamic therapy of cancer cells
A new near infrared photosensitizing nanoplatform containing blue-emitting up-conversion nanoparticles and hypocrellin A for photodynamic therapy of cancer cells
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一种含有蓝光上转换纳米颗粒和竹红菌素 A 的新型近红外光敏纳米平台，用于癌细胞的光动力治疗
DOI：
10.1039/c3nr03515h
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发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Zhao, Yuliang
中科院分区：
文献类型：
--
作者：
Jin, Shan;Zhou, Liangjun;Zhao, Yuliang
利用上转换纳米粒子（UCNPs）的光动力治疗（PDT）已经获得了显着的兴趣，由于其独特的能力，近红外光转换为紫外线/可见光。以前的工作主要集中在制备绿色和红色发光的UCNPs负载光敏剂（PS）的PDT。在这项工作中，我们首次开发了一种新的多功能纳米平台，将蓝光发射的UCNPs与蓝光激发的PS（竹红菌甲素，HA）相结合，作为癌细胞PDT的NIR光敏纳米平台。制备了Tween20包覆的NaYbF4：Tm，Gd@NaGdF4纳米颗粒（Tween20-UCNPs）作为PS载体，该纳米颗粒具有较强的蓝色上转换发光和良好的亲水性。蓝光发射带与HA的有效吸收带匹配良好，从而促进共振能量从UCNPs转移到HA，然后活化HA以产生单线态氧（O-1（2））。体外实验表明，Tween 20-UCNPs@HA复合物在980 nm近红外激发下能有效产生O-1（2），杀伤癌细胞。此外，这些含Gd 3+和Yb 3+的纳米颗粒在T-1加权磁共振成像（MRI）和计算机断层扫描（CT）成像中也表现出正对比效应，使其成为同时进行PDT和生物成像的多功能平台。
The utilization of up-conversion nanoparticles (UCNPs) for photodynamic therapy (PDT) has gained significant interest due to their unique ability to convert near infrared light to UV/visible light. Previous work mainly focused on the fabrication of green and red emitting UCNPs to load photosensitizers (PSs) for PDT. In this work, we firstly developed a new multifunctional nanoplatform combining blue-emitting UCNPs with blue-light excited PS (hypocrellin A, HA) as a NIR photosensitizing nanoplatform for PDT of cancer cells. Tween 20 coated NaYbF4:Tm, Gd@NaGdF4 UCNPs (Tween 20-UCNPs) with strong blue up-conversion luminescence and good water dispersibility were prepared for use as PS carriers. The blue emission band matched well with the efficient absorption band of HA, thereby facilitating the resonance energy transfer from UCNPs to HA and then activating HA to produce singlet oxygen (O-1(2)). The in vitro study showed that these Tween 20-UCNPs@HA complexes could efficiently produce O-1(2) to kill cancer cells under 980 nm NIR excitation. Moreover, these Gd3+ and Yb3+ containing nanoparticles also exhibited positive contrast effects in both T-1 weighted magnetic resonance imaging (MRI) and computed tomography (CT) imaging, making them become a multifunctional platform for simultaneous PDT and bio-imaging.