Identity and ecophysiology of uncultured actinobacterial polyphosphate-accumulating organisms in full-scale enhanced biological phosphorus removal plants
Identity and ecophysiology of uncultured actinobacterial polyphosphate-accumulating organisms in full-scale enhanced biological phosphorus removal plants
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DOI：
10.1128/aem.71.7.4076-4085.2005
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发表时间：
2005-07-01
影响因子：
4.4
通讯作者：
Nielsen, PH
中科院分区：
文献类型：
--
作者：
Kong, YH;Nielsen, JL;Nielsen, PH
采用显微放射自显影结合荧光原位杂交（MAR-FISH）技术在全规模强化生物除磷（EBPR）工厂中筛选潜在的聚磷菌（PAO）。结果表明，除了未培养的红环菌相关的PAO外，与革兰氏阳性放线菌的基因探针杂交的两种形态型也积极参与正磷酸盐（Pi）的吸收。克隆库分析和使用两种新的寡核苷酸探针进行的MAR-FISH进一步研究表明，两种形态型（四分体簇中的球菌和簇中的短棒）与放射细菌纲内孢子囊科的四球菌属关系相对密切（93%至98%）在其16 S rRNA基因中相似性）。FISH分析表明，以Actino-658为探针的短杆状菌数量最多，占细菌总数的12%，而以Actino-221为探针的四分体球菌数量最多，占7%。这两种形态型采取了P-I有氧只有当，在以前的厌氧阶段，他们采取了有机物从废水或氨基酸的混合物。它们不能摄取短链脂肪酸（例如，乙酸盐）、葡萄糖或乙醇。在厌氧期间产生的存储化合物是不是聚羟基链烷酸酯，红环菌相关的PAO，其身份仍然是未知的。P-1的生长和吸收发生在氧和硝酸盐的存在下，但不存在亚硝酸盐，表明缺乏分解能力。这些放线菌PAO的发生在10个全面的EBPR植物的调查显示，这两种形态广泛存在，并在几个植物更丰富的红环菌相关的PAO，从而发挥了非常重要的作用，在EBPR过程中。
Microautoradiography combined with fluorescence in situ hybridization (MAR-FISH) was used to screen for potential polyphosphate-accumulating organisms (PAO) in a full-scale enhanced biological phosphorus removal (EBPR) plant. The results showed that, in addition to uncultured Rhodocyclus-related PAO, two morphotypes hybridizing with gene probes for the gram-positive Actinobacteria were also actively involved in uptake of orthophosphate (Pi). Clone library analysis and further investigations by MAR-FISH using two new oligonucleotide probes revealed that both morphotypes, cocci in clusters of tetrads and short rods in clumps, were relatively closely related to the genus Tetrasphaera within the family Intrasporangiaceae of the Actinobacteria (93 to 98% similarity in their 16S rRNA genes). FISH analysis of the community biomass in the treatment plant investigated showed that the short rods (targeted by probe Actino-658) were the most abundant (12% of all Bacteria hybridizing with general bacterial probes), while the cocci in tetrads (targeted by probe Actino-221) made up 7%. Both morphotypes took up P-i aerobically only if, in a previous anaerobic phase, they had taken up organic matter from wastewater or a mixture of amino acids. They could not take up short-chain fatty acids (e.g., acetate), glucose, or ethanol under anaerobic or aerobic conditions. The storage compound produced during the anaerobic period was not polyhydroxyalkanoates, as for Rhodocyclus-related PAO, and its identity is still unknown. Growth and uptake of P-i took place in the presence of oxygen and nitrate but not nitrite, indicating a lack of denitrifying ability. A survey of the occurrence of these actinobacterial PAO in 10 full-scale EBPR plants revealed that both morphotypes were widely present, and in several plants more abundant than the Rhodocyclus-related PAO, thus playing a very important role in the EBPR process.