TOTAL NUMBER OF NEURONS AND GLIAL-CELLS IN HUMAN-BRAIN NUCLEI ESTIMATED BY THE DISECTOR AND THE FRACTIONATOR
TOTAL NUMBER OF NEURONS AND GLIAL-CELLS IN HUMAN-BRAIN NUCLEI ESTIMATED BY THE DISECTOR AND THE FRACTIONATOR
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DOI：
10.1111/j.1365-2818.1988.tb04582.x
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发表时间：
1988-04-01
影响因子：
2
通讯作者：
GUNDERSEN, HJG
中科院分区：
文献类型：
--
作者：
PAKKENBERG, B;GUNDERSEN, HJG
无偏估计的神经元和神经胶质细胞的总数从中央区域的灰质在人脑中获得使用的解剖原则的修改，这是不受石蜡包埋组织的组织学处理。截面厚度不进入估算器，也不需要知道。分析了各抽样水平对总细胞数估计量方差的贡献，设计了最优抽样方案。使用该方法，可以在不到1小时的时间内获得对特定脑区域中神经元总数的精确和无偏估计。
Unbiased estimates of the total number of neurons and glial cells from central regions of grey matter in human brains are obtained using the disector principle in modifications which are unaffected by the histological processing of paraffin-embedded tissue. Section thickness does not enter into the estimator and need not be known. An analysis of the contributions from all sampling levels to the variance of the estimator of total cell number has been used to design an optimal sampling scheme. Using this method, a precise and unbiased estimate of the total number of neurons in a defined brain region can be obtained in less than 1 h.