Radiofrequency coagulation of renal parenchyma: Comparison of effects of energy generators on treatment efficacy
Radiofrequency coagulation of renal parenchyma: Comparison of effects of energy generators on treatment efficacy
复制标题
DOI：
10.1089/089277902753619564
复制
发表时间：
2002-03-01
影响因子：
2.7
通讯作者：
Cadeddu, JA
中科院分区：
文献类型：
--
作者：
Gettman, MT;Lotan, Y;Cadeddu, JA
目的：我们比较了实验性肾脏病变的建立与温度为基础的或阻抗为基础的射频（RF）发生器使用正常的肾实质中的动物模型与协议由manufacturer.Materials和方法：双边肾动员完成在七个农场猪准备RF消融，和每个RF探头的尖叉扩大到2厘米直径。处理后立即（N = 3）或24小时（N = 4）处死猪。结果：无论使用何种射频仪，大体病变均为坚实的白色伴出血边缘。H&E染色显示两种RF系统均保留了肾结构，但丢失了不同的细胞质特征。NADH染色显示在由任一RF系统产生的损伤内没有活细胞。即刻和24小时存活后，基于温度的射频发生器治疗病灶的平均尺寸分别为1.6 X 1.8 X 1.3 cm和2.0 X 1.6 X 1.9 cm，基于阻抗的射频发生器分别为2.7 X 2.4 X 2.5 cm和2.2 X 2.1 X 1.8 cm。在猪模型中，使用特定的射频能量发生器和推荐的治疗方案似乎不会影响正常肾实质的凝血。
Purpose: We compared experimental renal lesions created with temperature-based or impedance-based radiofrequency (RF) generators using normal renal parenchyma in an animal model with protocols recommended by the manufacturer.Materials and Methods: Bilateral renal mobilization was completed in seven farm pigs in preparation for RF ablation, and the tines of each RF probe were expanded to a 2-cm diameter. Pigs were sacrificed immediately (N = 3) or at 24 hours (N = 4) after treatment. Lesions were examined grossly and microscopically after hematoxylin and eosin (H&E) and nicotinamide adenine dinucleotide (NADH) staining.Results: Gross lesions were firm and white with a hemorrhagic border, regardless of RF generator. Staining with H&E revealed preservation of renal architecture but loss of distinct cytoplasmic features with both RF systems. Staining for NADH showed no viable cells within the lesions produced by either RF system. Immediately and after a 24-hour survival, the mean sizes of the treatment lesions were 1.6 X 1.8 X 1.3 cm and 2.0 X 1.6 X 1.9 cm, respectively, for the temperature-based RF generator and 2.7 X 2.4 X 2.5 cm and 2.2 X 2.1 X 1.8 cm, respectively, for the impedance-based RF generator.Conclusion: Use of a specific RF energy generator with recommended treatment protocols does not appear to affect coagulation of normal renal parenchyma in the porcine model.