Differential effect of poly rI.rC and Newcastle disease virus on the expression of interferon and cellular genes in mouse cells.
Differential effect of poly rI.rC and Newcastle disease virus on the expression of interferon and cellular genes in mouse cells.
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聚rI.rC和新城疫病毒对小鼠细胞中干扰素和细胞基因表达的差异作用。
DOI：
10.1016/0042-6822(85)90140-0
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Pitha,PM
中科院分区：
文献类型：
--
作者：
Kelley,KA;Pitha,PM
在聚 rI·rC 诱导和新城疫病毒 (NDV) 感染的小鼠细胞中检查了 I 型鼠干扰素 (MuIFN) 基因和其他几种细胞基因的表达。对诱导的 L 细胞的 RNA 进行 Northern 分析表明，MuIFN-α 在 NDV 感染的细胞中有效表达，但在聚 rI·rC 诱导的细胞中仅表达低水平。然而，MuIFN-β1 在经聚 rI·rC 处理或感染新城疫病毒的细胞中同样表达良好。正如使用聚 rI·rC 特异性探针所显示的，干扰素诱导与细胞摄取聚 rI·rC 相关。细胞中α和β1mRNA的相对水平在诱导后10小时达到最大值，这表明α和β1干扰素基因的协调表达。还检查了病毒感染对干扰素共诱导的两种小鼠基因（pMIF20/11和pMIF3/10）和几种细胞基因表达的影响。 pMIF20/11 是诱导型基因，而 pMIF3/10 基因在小鼠 L 细胞中组成型表达。病毒感染增强了 pMIF3/10 基因以及其他两个细胞基因的表达，但聚 rI·rC 处理则没有。然而，H-2和c-myc，β-肌动蛋白基因的表达没有改变。这些数据表明，病毒感染细胞中基因表达的增强并不限于干扰素系统。
The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI·rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-αs are expressed efficiently in NDV infected cells but only at low levels in poly rI·rC induced cells. MuIFN-β1, however, is expressed equally well in cells treated with poly rI·rC or infected with NDV. As shown by the use of a probe specific for poly rI·rC, interferon induction correlates with the cellular uptake of poly rI·rC into the cells. The relative levels of α andβ1mRNAs in the cells reached a maximum at 10 hr after the induction which indicates coordinate expression of α andβ1interferon genes. The effect of viral infection on the expression of two murine genes coinduced with interferon (pMIF20/11 and pMIF3/10) and several cellular genes was also examined. While pMIF20/11 is an inducible gene, the pMIF3/10 gene is expressed constitutively in mouse L cells. Viral infection, but not poly rI·rC treatment, enhanced the expression of the pMIF3/10 gene, as well as two other cellular genes; H-2 and c-myc, however, the expression of β-actin gene was unaltered. These data indicate that enhancement of gene expression in virus infected cells in not limited to the interferon system.