Plasmid-mediated bioaugmentation of activated sludge bacteria in a sequencing batch moving bed reactor using pNB2
Plasmid-mediated bioaugmentation of activated sludge bacteria in a sequencing batch moving bed reactor using pNB2
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DOI：
10.1111/j.1472-765x.2005.01754.x
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发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Hausner, M
中科院分区：
文献类型：
--
作者：
Bathe, S;Schwarzenbeck, N;Hausner, M
目的：质粒pNB 2的生物强化的细菌在模型污水处理反应器中接受3-氯苯胺（3-CA）的适用性进行了调查。方法和结果：设置三个生物膜反应器进行了研究，所有最初接种的活性污泥细菌。反应器PB接受不能降解3-CA的恶臭假单胞菌pNB 2供体菌株。阳性对照反应器P接受3-CA降解睾丸酮丛毛单胞菌pNB 2-转移接合子。阴性对照反应器N保持不变。反应器P显示3-CA从实验开始降解，而在反应器PB中，降解在初始滞后期后开始。在反应器N中未观察到降解。PCR分析表明，恶臭假单胞菌供体丰度在反应器PB中下降，而质粒丰度没有下降，表明转移到其他细菌。对几种不同的3-CA降解菌C.结论：成功实现了质粒介导的C. testosteroni是占主导地位的3-CA降解pNB 2 transconjugant物种活跃在反应器PB。意义和影响的研究：这项研究强调了基因转移的潜力，以促进建立和传播的遗传信息一般，特别强调传播的异化基因的传播异生物质降解潜力。
Aims: The applicability of plasmid pNB2 for bioaugmentation of bacteria in model wastewater treatment reactors receiving 3-chloroaniline (3-CA) was investigated.Methods and Results: A setup of three biofilm reactors was studied, all initially inoculated with bacteria from activated sludge. Reactor PB received a Pseudomonas putida pNB2 donor strain not able to degrade 3-CA. Positive control reactor P received a 3-CA degrading Comamonas testosteroni pNB2-transconjugant. The negative control reactor N remained unchanged. Reactor P showed 3-CA degradation from the beginning of the experiment whereas in reactor PB, degradation started after an initial lag period. No degradation was observed in reactor N. PCR analysis showed that the P. putida donor abundance dropped in reactor PB, whereas the plasmid abundance did not, indicating transfer to other bacteria. A number of different 3-CA degrading C. testosteroni strains carrying pNB2 could be isolated from reactor PB.Conclusions: A successful plasmid-mediated bioaugmentation was achieved with C. testosteroni being the dominant 3-CA degrading pNB2 transconjugant species active in reactor PB.Significance and Impact of the Study: The study underlines the potential of gene transfer to contribute to establishment and spread of genetic information in general, particularly emphasizing the spread of xenobiotic degrading potential by dissemination of catabolic genes.