Novel insight into the detailed myocardial motion and deformation of the rodent heart using high-resolution phase contrast cardiovascular magnetic resonance.
Novel insight into the detailed myocardial motion and deformation of the rodent heart using high-resolution phase contrast cardiovascular magnetic resonance.
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DOI：
10.1186/1532-429x-15-82
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发表时间：
2013-09-14
期刊：
影响因子：
--
通讯作者：
Sjaastad I
中科院分区：
文献类型：
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作者：
Espe EK;Aronsen JM;Skårdal K;Schneider JE;Zhang L;Sjaastad I
心血管磁共振（PC-CMR）是一种功能强大的多功能工具，可以评估在体心肌运动。然而，PC-CMR对运动相关伪影敏感，导致几何系统性错误，从而使心肌功能的区域分析具有挑战性。本研究的目的是建立一种优化的PC-CMR方法，能够提供新的见解，在复杂的区域运动和应变的啮齿动物心肌，并提供一个概念验证，在正常和患病大鼠心脏具有更高的时间和空间分辨率比以前报道的。建立了一种优化的PC-CMR方案，用于评估具有高时空分辨率的大鼠心肌的运动和变形，并对10只具有不同程度心功能不全的动物进行了检查，并作为概念验证。计算整体和局部心肌速度和周向应变，并将结果与5只对照动物进行比较。此外，根据斑点追踪超声心动图验证了全局应变测量，并评价了研究方案的研究间和研究内变异性。所提出的方法可以评估局部心肌功能的大鼠与高层次的细节，时间分辨率为3.2毫秒，并使用32个圆周段进行分析。在功能障碍的心脏中，整体和局部功能明显改变，包括整体峰值降低，局部异质性增加和不同步指数增加。从PC-CMR数据得出的应变与超声心动图非常一致（r = 0.95，p < 0.001;一致性限-0.02 ± 3.92%应变），速度和应变的研究内和研究间变异性均较低（一致性限度，径向运动：0.01 ± 0.32 cm/s和−0.06 ± 0.75 cm/s;周向应变：-0.16 ± 0.89%应变和−0.71 ± 1.67%应变，分别用于研究内和研究间）。我们证明，第一次，PC-CMR使高分辨率的评价在体内的圆周应变除了心肌运动的大鼠心脏。结合CMR的上级几何稳健性，这最终提供了一个工具，纵向研究啮齿动物的区域功能的高水平的细节。
Phase contrast velocimetry cardiovascular magnetic resonance (PC-CMR) is a powerful and versatile tool allowing assessment of in vivo motion of the myocardium. However, PC-CMR is sensitive to motion related artifacts causing errors that are geometrically systematic, rendering regional analysis of myocardial function challenging. The objective of this study was to establish an optimized PC-CMR method able to provide novel insight in the complex regional motion and strain of the rodent myocardium, and provide a proof-of-concept in normal and diseased rat hearts with higher temporal and spatial resolution than previously reported. A PC-CMR protocol optimized for assessing the motion and deformation of the myocardium in rats with high spatiotemporal resolution was established, and ten animals with different degree of cardiac dysfunction underwent examination and served as proof-of-concept. Global and regional myocardial velocities and circumferential strain were calculated, and the results were compared to five control animals. Furthermore, the global strain measurements were validated against speckle-tracking echocardiography, and inter- and intrastudy variability of the protocol were evaluated. The presented method allows assessment of regional myocardial function in rats with high level of detail; temporal resolution was 3.2 ms, and analysis was done using 32 circumferential segments. In the dysfunctional hearts, global and regional function were distinctly altered, including reduced global peak values, increased regional heterogeneity and increased index of dyssynchrony. Strain derived from the PC-CMR data was in excellent agreement with echocardiography (r = 0.95, p < 0.001; limits-of-agreement −0.02 ± 3.92%strain), and intra- and interstudy variability were low for both velocity and strain (limits-of-agreement, radial motion: 0.01 ± 0.32 cm/s and −0.06 ± 0.75 cm/s; circumferential strain: -0.16 ± 0.89%strain and −0.71 ± 1.67%strain, for intra- and interstudy, respectively). We demonstrate, for the first time, that PC-CMR enables high-resolution evaluation of in vivo circumferential strain in addition to myocardial motion of the rat heart. In combination with the superior geometric robustness of CMR, this ultimately provides a tool for longitudinal studies of regional function in rodents with high level of detail.