Detection of human immunodeficiency virus type 1 Nef and CD4 physical interaction in living human cells by using bioluminescence resonance energy transfer
Detection of human immunodeficiency virus type 1 Nef and CD4 physical interaction in living human cells by using bioluminescence resonance energy transfer
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DOI：
10.1128/jvi.79.13.8629-8636.2005
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Aubertin, AM
中科院分区：
文献类型：
--
作者：
Cluet, D;Bertsch, C;Aubertin, AM
人类免疫缺陷病毒1型（HIV-1）Nef蛋白下调CD 4是病毒毒力的关键功能。这种活性可以通过直接Nef-CD 4相互作用介导。我们研究了形成，在原位，这样的蛋白质之间的复合物，使用生物发光共振能量转移技术和免疫共沉淀。我们的数据清楚地表明Nef和CD 4在完整的人类细胞中相互作用。此外，我们的研究结果清楚地表明，CD 4胞质结构域的双亮氨酸基序，Nef诱导的CD 4下调的关键，是没有牵连的Nef/CD 4复合物的形成在细胞环境中。
CD4 down-regulation by human immunodeficiency virus type 1 (HIV-1) Nef protein is a key function for virus virulence. This activity may be mediated by a direct Nef-CD4 interaction. We investigated the formation, in situ, of such a complex between proteins using bioluminescence resonance energy transfer technology and coimmunoprecipitations. Our data clearly demonstrate that Nef and CD4 interact in intact human cells. Moreover, our results clearly indicate that the dileucine motif of the CD4 cytoplasmic domain, critical for the Nef-induced CD4 down-regulation, is not implicated in the Nef/CD4 complex formation in the cellular context.