Development and comparison of biosensors for in-vivo applications.
Development and comparison of biosensors for in-vivo applications.
复制标题
用于体内应用的生物传感器的开发和比较。
作者：
D. Georganopoulou;R. Carley;Deborah A. Jones;M. Boutelle
电化学生物传感器越来越受到人们的关注，特别是那些被开发为直接应用于诊断领域，如神经科学的传感器。我们一直有兴趣开发一系列生物传感器，用于监测葡萄糖，乳酸，丙酮酸和谷氨酸，以在线研究实验室中的脑功能和监测神经重症监护中的脑健康。为了使生物传感器在这些情况下有效地发挥作用，它必须联合收割机以下特性：快速响应和高灵敏度，良好的再现性和足够的稳定性。在这项研究中，我们比较了多种不同电流生物传感器策略的性能。这些包括固定化酶的二茂铁介导（系统A），基于氧化还原水凝胶的系统（系统B），和使用聚苯胺的导电聚合物方法（系统C）。如果需要的话，所有测定都作为具有上游固定化酶床的流动注射系统操作。当对H2O2系统A和B进行校准时，反应速度足够快，可提供高达0.2 mM的定量转化率。在此浓度以上，响应受到辣根过氧化物酶动力学和最终酶负载的限制。系统C显示出受限的H2O2响应。当校准葡萄糖（通过使用固定的葡萄糖氧化酶）系统B表现出最高的灵敏度，但其分析范围受到限制，因为该系统成为有限的H2O2响应。与H2O2和系统B相比，系统A对分析物的灵敏度较低，但有用范围较大。介质之间的固定化酶循环的问题进行了讨论。系统C给出了极好的线性范围，但灵敏度受到背景噪声的限制。还描述了系统的稳定性和再现性。总之，从这项研究中，二茂铁系统被证明是最有用的，现在已被用于第一个双重在线监测葡萄糖和乳酸盐的患者在神经重症监护。
Electrochemical biosensors have been of increasing interest, especially those developed to be directly applied in diagnostic areas, such as neuroscience. We have been interested in developing a range of biosensors for monitoring glucose, lactate, pyruvate, and glutamate in order to study on-line both brain function in the laboratory and to monitor brain health in neurointensive care. For a biosensor to function effectively in these situations, it has to combine the following characteristics: quick response and high sensitivity, good reproducibility and adequate stability. In this study we compared the performance of a number of different amperometric biosensors strategies. These included ferrocene mediation of immobilised enzymes (system A), a redox hydrogel based system (system B), and a conducting polymer approach using polyaniline (system C). All assays were operated as flow-injection systems with upstream immobilised enzyme beds if necessary. When calibrated for H2O2 systems A and B reacted quickly enough to give quantitative conversion up to 0.2 mM. Above this concentration the response was limited by horseradish peroxidase enzyme kinetics and eventually enzyme loading. System C showed a restricted H2O2 response. When calibrated for glucose (by use of immobilised glucose oxidase) system B exhibited the highest sensitivity but its analytical range was restricted because the system became limited by H2O2 response. System A had low sensitivity for analyte compared to H2O2 and system B, but a greater useful range. Problems of mediator cycling between the immobilised enzymes are discussed. System C gave an excellent linear range but sensitivity was limited by background noise. Stability and reproducibility of the systems are also described. In conclusion, from this study the ferrocene system proved to be overall most useful and has now been used in the first dual on-line monitoring of glucose and lactate in patients in neurointensive care.
影响因子：
7.4
作者：
Kulagina, NV;Shankar, L;Michael, AC
通讯作者：
Michael, AC
影响因子：
7.4
作者：
GARGUILO, MG;MICHAEL, AC
通讯作者：
MICHAEL, AC