Effects of hyperbaric oxygen and tendon-bone integration treatment on tendon graft in bone tunnel: Biochemical and histological analysis in rabbits
Effects of hyperbaric oxygen and tendon-bone integration treatment on tendon graft in bone tunnel: Biochemical and histological analysis in rabbits
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DOI：
10.1002/jor.20360
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发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Ueng, Steve W. N.
中科院分区：
文献类型：
--
作者：
Yeh, Wen-Ling;Lin, Song-Shu;Ueng, Steve W. N.
尽管在临床应用中取得了一定的成功，但用于前交叉韧带（ACL）重建的肌腱移植物的结果仍然不令人满意。本研究观察了高压氧（HBO）对兔肌腱-骨连接处新生血管、肌腱移植物胶原纤维和肌腱移植物-骨界面纳入骨隧道的影响。将40只家兔分为两组。HBO组在2.5个大气压下暴露于100%氧气中，每天2 h，每周连续暴露5天。对照组饲养在暴露于正常空气的笼中。在术后6、12和18周对12只家兔进行组织学研究。对24只家兔在术后12周和18周进行生物力学研究。术后18周，对4只家兔进行电子显微镜（EM）分析。实验结果表明，高压氧组比对照组有更多的Sharpey纤维桥接新形成的纤维软骨和移植物。此外，HBO治疗增加了新生血管的形成，并增强了肌腱移植物与骨之间的渐进性界面的结合。生物力学分析表明，高压氧组的最大拔出力高于对照组。电镜观察显示高压氧处理后再生胶原纤维致密、规整。根据实验结果，HBO治疗是一种可能改善ACL重建术后结局的治疗方式。(c)2007骨科研究学会。
Despite moderate success in clinical applications, outcome of tendon grafts employed for anterior cruciate ligament (ACL) reconstruction remains unsatisfactory. This study investigated the effects of hyperbaric oxygen (HBO) on neovascularization at the tendon-bone junction, Collagen fibers of the tendon graft, and the tendon graft-bony interface incorporated into the osseous tunnel in rabbits. Forty rabbits were assigned to two groups. The HBO group was exposed to 100% oxygen at 2.5 atmospheres pressure for 2 h daily, 5 consecutive days in a week. The control group was maintained in cages exposed to normal air. Histological studies of 12 rabbits were performed postoperatively at 6, 12, and 18 weeks. Biornechanical studies of 24 rabbits were conducted postoperatively at 12 and 18 weeks. Electron microscopy (EM) analyses of four rabbits were performed postoperatively at 18 weeks. Experimental results demonstrated that a higher number of Sharpey's fibers bridged the newly formed fibrocartilage and graft in the HBO group than in the control group. In addition, HBO treatment increased neovascularization and enhanced the incorporation of the progressive interface between tendon graft and bone. Biornechanical analysis showed that the HBO group achieved higher maximal pullout strength than the control group. Examination by EM showed that HBO treatment resulted in regenerated Collagen fibers with increased compaction and regularity. Based on experimental results, HBO treatment is a treatment modality that potentially improves outcome following ACL reconstruction. (c) 2007 Orthopaedic Research Society.