Enamel factors regulate expression of genes associated with cementoblasts.
Enamel factors regulate expression of genes associated with cementoblasts.
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DOI：
10.1902/jop.2000.71.12.1829
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发表时间：
2000-12
影响因子：
4.3
通讯作者：
Yoshihiko Tokiyasu;Takashi Takata;E. Saygin;M. J. Somerman
中科院分区：
文献类型：
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作者：
Yoshihiko Tokiyasu;Takashi Takata;E. Saygin;M. J. Somerman
背景技术为了设计可预测的牙周再生疗法，了解局部环境内的细胞对被视为有吸引力的候选因素的反应性非常重要。本研究的目的是确定牙釉质基质衍生物 (EMD) 对体外和体内成牙骨质细胞行为的影响。方法采用骨钙素（OC）启动子SV40转基因小鼠获得成牙骨质细胞。为了进行比较，使用了来自这些小鼠的前成骨细胞以及另一种小鼠前成骨细胞系MC3T3-E1细胞。通过细胞计数评估暴露于 EMD 的细胞的以下变化：1) 8 天期间的增殖； 2)使用Northern印迹分析进行基因表达； 3) 通过 von Kossa 染色在体外进行生物矿化，并通过从免疫缺陷 (SCID) 小鼠中取出的植入物制备组织学样品，其中成牙骨质细胞在植入前用 EMD 进行处理。结果 EMD 促进所有细胞类型的增殖。第 8 天时，EMD 下调成牙骨质细胞和 MC3T3-E1 细胞中的骨钙素转录物，并显着上调 MC3T3-E1 细胞中的骨桥蛋白基因表达，在成牙骨质细胞中轻微上调。在体外，EMD 降低了成牙骨质细胞介导的生物矿化。相比之下，从 SCID 小鼠中取出的植入物中发现了矿化，其中细胞经过 EMD 预处理。结论这些结果表明EMD可以影响成牙骨质细胞和成骨细胞的活性，因此可能能够调节牙周再生部位的细胞活性。
BACKGROUND In order to design predictable periodontal regenerative therapies, it is important to understand the responsiveness of cells within the local environment to factors considered attractive candidates. The aim of this study was to determine the effect of an enamel matrix derivative (EMD) on cementoblast behavior in vitro and in vivo. METHODS Osteocalcin (OC) promoter SV40 transgenic mice were used to obtain cementoblasts. For comparison, preosteoblasts from these mice, as well as another murine pre-osteoblast cell line, MC3T3-E1 cells, were used. Cells exposed to EMD were evaluated for changes in: 1) proliferation over an 8-day period by cell counting; 2) gene expression using Northern blot analysis; and 3) biomineralization by von Kossa stain, in vitro and by preparing histological samples from implants retrieved from immunodeficient (SCID) mice, where cementoblasts were treated with EMD prior to implantation. RESULTS EMD promoted proliferation of all cell types. EMD down-regulated osteocalcin transcripts in cementoblasts and MC3T3-E1 cells and up-regulated osteopontin gene expression markedly in MC3T3-E1 cells and slightly in cementoblasts at day 8. In vitro, EMD decreased cementoblast-mediated biomineralization. In contrast, mineralization was noted in implants retrieved from SCID mice, where cells were pretreated with EMD. CONCLUSION These results indicate that EMD can influence activities of cementoblasts and osteoblasts, and thus may be able to regulate cell activities at a periodontal regenerative site.