THE SPIN-TRAPPING OF SUPEROXIDE AND HYDROXYL FREE-RADICALS WITH DMPO (5,5-DIMETHYLPYRROLINE-N-OXIDE) - MORE ABOUT IRON
THE SPIN-TRAPPING OF SUPEROXIDE AND HYDROXYL FREE-RADICALS WITH DMPO (5,5-DIMETHYLPYRROLINE-N-OXIDE) - MORE ABOUT IRON
复制标题
DOI：
10.3109/10715769309056s79
复制
发表时间：
1993-01-01
期刊：
影响因子：
--
通讯作者：
BUETTNER, GR
中科院分区：
文献类型：
--
作者：
BUETTNER, GR
超氧化物与自旋捕获剂DMPO（5，5-二甲基吡咯啉-N-氧化物）的反应被广泛用于研究超氧化物的产生以及与超氧化物相关的羟基自由基形成相关的问题。然而，在电子顺磁共振自旋捕获实验中观察到的DMPO/.OOH和DMPO/.OH信号强度的解释并非没有困难。在本文中，我报告的实验表明：1。在DMPO自旋捕获实验中，超氧化物形成的通量可以改变弱DMPO/.OH信号的表观重要性;铁对DMPO/.OOH自旋捕获结果有影响; DMPO/.OOH很少自发分解形成DMPO/. OH。
The reaction of superoxide with the spin trap DMPO (5,5-dimethylpyrroline-N-oxide) is widely used to study superoxide production as well as issues associated with the superoxide-related formation of hydroxyl radical. However, the interpretation of observed intensities of DMPO/.OOH and DMPO/.OH signals in electron paramagnetic resonance spin trapping experiments is not without its difficulties. In this paper, I report experiments that demonstrate: 1. That the flux of superoxide formation in a DMPO spin trapping experiment can alter the apparent importance of weak DMPO/.OH signals; 2. That iron can influence the DMPO/.OOH spin trapping results; 3. That there is very little spontaneous breakdown of DMPO/.OOH to form DMPO/.OH.