Preparation of β‐cyclodextrin‐gold nanoparticles modified open tubular column for capillary electrochromatographic separation of chiral drugs
Preparation of β‐cyclodextrin‐gold nanoparticles modified open tubular column for capillary electrochromatographic separation of chiral drugs
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DOI：
10.1002/elps.201700462
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发表时间：
2018-04
期刊：
影响因子：
2.9
通讯作者：
Li Zhou;Shenmeng Jiang;Xue Zhang;Linlin Fang;Xingjie Guo
中科院分区：
文献类型：
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作者：
Li Zhou;Shenmeng Jiang;Xue Zhang;Linlin Fang;Xingjie Guo
制备了β-环糊精（β-CD）修饰纳米金（AuNPs）毛细管电色谱柱（OT柱）。通过在3-巯丙基三甲氧基硅烷（MPTMS）预衍生毛细管上自组装金纳米粒子，然后用巯基β-环糊精（SH-β-CD）修饰，构建了开管柱。利用扫描电子显微镜（SEM）、透射电子显微镜（TEM）和紫外可见光谱对所制备的开管柱和所合成的金纳米粒子进行了表征。通过比较不同包被次数的纳米金和硫醇类β-环糊精，得到了制备开管柱的最佳条件。同时，对缓冲液pH、缓冲液浓度、分离电压等分离参数进行了优化。通过联苯苄唑、非索非那定、奥美拉唑和兰索拉唑4对药物对映体的分离，验证了开管柱的分离效果，获得了满意的分离结果。该色谱柱具有良好的稳定性和重复性。通过日内、日间和柱间研究获得的相对标准偏差值小于5%。
In this paper, β‐cyclodextrin (β‐CD) modified gold nanoparticles (AuNPs) coated open tubular column (OT column) was prepared for capillary electrochromatography. The open tubular column was constructed through self‐assembly of gold nanoparticles on 3‐mercaptopropyl‐trimethoxysilane (MPTMS) prederivatized capillary and subsequent modification of thiols β‐cyclodextrin (SH‐β‐CD). Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and ultraviolet visible spectroscopy were carried out to characterize the prepared open tubular column and synthesized gold nanoparticles. By comparing different coating times of gold nanoparticles and thiols β‐cyclodextrin, we got the optimal conditions for preparing the open tubular column. Also, the separation parameters were optimized including buffer pH, buffer concentration and applied voltage. Separation effectiveness of open tubular column was verified by the separation of four pairs of drug enantiomers including bifonazole, fexofenadine, omeprazole and lansoprazole, and satisfactory separation results were achieved for these analytes studied. In addition, the column showed good stability and repeatability. The relative standard deviation values less than 5% were obtained through intra‐day, inter‐day, and column‐to‐column investigations.