Enzymatically modified isoquercitrin promotes energy metabolism through activating AMPKα in male C57BL/6 mice
Enzymatically modified isoquercitrin promotes energy metabolism through activating AMPKα in male C57BL/6 mice
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DOI：
10.1039/c9fo01008d
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发表时间：
2019-08-01
期刊：
影响因子：
6.1
通讯作者：
Ashida, Hitoshi
中科院分区：
文献类型：
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作者：
Jiang, Hao;Yoshioka, Yasukiyo;Ashida, Hitoshi
槲皮素具有多种保健功能，但其生物利用度低限制了这些功能。酶促改性异槲皮苷（EMIQ）是一种生物利用度比槲皮素高的槲皮素糖苷。在这项研究中，我们研究了EMIQ是否调节小鼠的能量代谢及其潜在的分子机制。雄性C57 BL/6小鼠用不同剂量的EMIQ或槲皮素（0.02%、0.1%和0.5%）喂养正常饮食两周。补充0.1%EMIQ显著降低了白色脂肪组织（WAT）重量。补充0.02%和0.1%EMIQ促进WAT、肝脏和肌肉中腺苷酸活化蛋白激酶（AMPK）的磷酸化。在WAT中，0.1%EMIQ下调过氧化物酶体增殖物激活受体（PPAR）γ、CCAAT增强子结合蛋白（C/EBP）α、C/EBP β和固醇调节元件结合蛋白1的表达，以及上调线粒体解偶联蛋白（UCP）2和肉毒碱棕榈酰转移酶-1的表达。补充0.1%EMIQ还促进了WAT中的产热相关因子的表达，包括PPAR γ辅激活因子α（PGC-1 α）、UCP 1、含PR结构域的蛋白16和sirtuin 1。在肝脏中，EMIQ促进乙酰辅酶A羧化酶的磷酸化，并增加过氧化物酶体增殖物激活受体α、组成型雄烷受体和法尼醇X受体的表达。此外，补充0.02%或0.1%EMIQ可抑制血糖水平，并伴有葡萄糖转运蛋白4移位至肌肉质膜。我们的研究结果表明，EMIQ是一种潜在的食品添加剂，通过AMPK磷酸化调节能量代谢。
Quercetin possesses various health beneficial functions, but its poor bioavailability limits these functions. Enzymatically modified isoquercitrin (EMIQ) is a quercetin glycoside with a greater bioavailability than quercetin. In this study, we investigated whether EMIQ regulates energy metabolism in mice and its underlying molecular mechanism. Male C57BL/6 mice were fed a normal diet with different doses of EMIQ or quercetin (0.02%, 0.1% and 0.5%) for two weeks. Supplementation with 0.1% EMIQ significantly decreased white adipose tissue (WAT) weight. Supplementation with 0.02% and 0.1% EMIQ promoted phosphorylation of adenosine monophosphate activated protein kinase (AMPK) in the WAT, liver, and muscle. In the WAT, 0.1% EMIQ downregulated peroxisome proliferator-activated receptor (PPAR)gamma, CCAAT-enhancer-binding protein (C/EBP)alpha, C/EBP beta, and sterol regulatory element-binding protein 1 expression, as well as upregulated mitochondrial uncoupling protein (UCP) 2 and carnitine palmitoyltransferase-1 expression. Supplementation with 0.1% EMIQ also promoted the expression of thermogenesis-associated factors including PPAR gamma coactivator alpha (PGC-1 alpha), UCP1, PR-domain containing protein 16, and sirtuin 1 in the WAT. In the liver, EMIQ promoted the phosphorylation of acetyl-CoA carboxylase, and increased the expression of PPAR alpha, constitutive androstane-receptor, and farnesoid X receptor. Furthermore, supplementation with 0.02% or 0.1% EMIQ suppressed the plasma glucose level accompanied by the translocation of glucose transporter 4 to the plasma membrane of the muscle. Our results suggest that EMIQ is a potential food additive for the regulation of energy metabolism through AMPK phosphorylation.