Clonal Restriction of Platelet-associated Anti-GPIIb/IIIa Autoantibodies in Patients with Chronic ITP
Clonal Restriction of Platelet-associated Anti-GPIIb/IIIa Autoantibodies in Patients with Chronic ITP
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DOI：
10.1055/s-0037-1615754
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发表时间：
2001-05
影响因子：
6.7
通讯作者：
R. Mcmillan;J. Lopez-Dee;R. Bowditch
中科院分区：
文献类型：
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作者：
R. Mcmillan;J. Lopez-Dee;R. Bowditch
慢性免疫性血小板减少性紫癜是由于抗血小板表面抗原的自身抗体引起的血小板破坏。先前的研究表明，一些血清自身抗体是轻链限制性的，表明克隆起源。由于来自同一患者的血浆和血小板相关抗体可能结合不同的表位，因此评价血小板相关抗体的克隆性是重要的。对28例ITP患者的血小板相关自身抗体进行了研究。在直接检测的23种血小板相关抗体中，与血浆IgG轻链分布相比，16种显示出显著的轻链限制（7种完全限制和9种部分限制）。同样，12个血小板相关抗体洗脱液中有9个是轻链限制性的，5个是完全的，4个是部分的。在研究同一患者的血小板相关抗体和抗体IgM的所有病例中，结果一致。我们的结论是，一个显着的比例血小板相关抗体从ITP患者表现出明显的克隆性，轻链限制性评价。这些结果与ITP中的其他研究一致，表明抗原库有限。
Summary Chronic immune thrombocytopenic purpura is due to platelet destruction induced by autoantibodies against platelet surface antigens. Prior studies show that some serum autoantibodies are light-chain restricted, suggesting a clonal origin. Since plasma and platelet-associated antibody from the same patient may bind to different epitopes, it is important to evaluate the clonality of platelet-associated antibody. Platelet-associated autoantibodies from 28 ITP patients were studied. Of 23 platelet-associated antibodies tested directly, 16 showed significant light chain restriction (7 complete and 9 partial) when compared to plasma IgG light chain distribution. Similarly, 9 of 12 platelet-associated antibody eluates were light chain restricted, 5 complete and 4 partial. In all cases where platelet-associated antibody and antibody eluate from the same patient were studied, the results were concordant. We conclude that a significant proportion of platelet-associated antibodies from ITP patients show apparent clonality, as evaluated by light chain restriction. These results are consistent with other studies in ITP suggesting a limited antigenic repertoire.