Biocompatibility of calcium phosphate bone cement with optimized mechanical properties.
Biocompatibility of calcium phosphate bone cement with optimized mechanical properties.
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DOI：
10.1002/jbm.b.33370
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Clarke SA
中科院分区：
文献类型：
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作者：
Palmer I;Nelson J;Schatton W;Dunne NJ;Buchanan FJ;Clarke SA
这项工作的主要目的是研究和优化用于椎体成形术的磷酸钙骨水泥（CPC）的性能，以实现脊柱骨折的有效一期固定。胶原蛋白，牛和从海绵（肾形软骨），到CPC的掺入进行了研究。CPC和胶原-CPC复合材料的生物学特性通过使用人骨髓基质细胞进行体外评估。分别使用乳酸脱氢酶、PicoGreen和碱性磷酸酶活性测定评价细胞毒性、增殖和成骨细胞分化。添加两种类型的胶原蛋白导致细胞毒性增加，尽管未达到临床相关水平。1、7和14天后的细胞增殖没有变化。通过添加牛胶原蛋白，CPC的成骨潜力降低，但在添加海洋胶原蛋白的情况下保持不变。这些发现，加上以前的工作表明，以这种方式掺入海洋胶原蛋白可以改善CPC的物理性能，表明这种复合材料可以提供替代CPC的应用中，低凝固时间和更高的机械稳定性是重要的。版权所有© 2015作者.生物医学材料研究杂志B部分：应用生物材料，由Wiley期刊公司出版。104B：308-315，2015.
The broad aim of this work was to investigate and optimize the properties of calcium phosphate bone cements (CPCs) for use in vertebroplasty to achieve effective primary fixation of spinal fractures. The incorporation of collagen, both bovine and from a marine sponge (Chondrosia reniformis), into a CPC was investigated. The biological properties of the CPC and collagen–CPC composites were assessed in vitro through the use of human bone marrow stromal cells. Cytotoxicity, proliferation, and osteoblastic differentiation were evaluated using lactate dehydrogenase, PicoGreen, and alkaline phosphatase activity assays, respectively. The addition of both types of collagen resulted in an increase in cytotoxicity, albeit not to a clinically relevant level. Cellular proliferation after 1, 7, and 14 days was unchanged. The osteogenic potential of the CPC was reduced through the addition of bovine collagen but remained unchanged in the case of the marine collagen. These findings, coupled with previous work showing that incorporation of marine collagen in this way can improve the physical properties of CPCs, suggest that such a composite may offer an alternative to CPCs in applications where low setting times and higher mechanical stability are important. © 2015 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. 104B:308–315, 2015.