New insights into the mycorrhizal Rhizoscyphus ericae aggregate: spatial structure and co-colonization of ectomycorrhizal and ericoid roots
New insights into the mycorrhizal Rhizoscyphus ericae aggregate: spatial structure and co-colonization of ectomycorrhizal and ericoid roots
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DOI：
10.1111/j.1469-8137.2010.03353.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Anderson, Ian C.
中科院分区：
文献类型：
--
作者：
Grelet, Gwen-Aelle;Johnson, David;Anderson, Ian C.
P>从共存的杜鹃花灌木和外生菌根树的根部回收了杜鹃根霉聚集体中的真菌。然而，迄今为止，没有证据表明相同的个体基因型定植于两个宿主，也没有关于可能形成的菌丝网络范围的信息。使用空间明确的核心采样，我们从邻近的樟子松（外生菌根）和越橘（杜鹃花菌根）根中分离出真菌，并应用简单间序列重复（ISSR）分型来评估共享基因的发生和范围。大多数分离株均已鉴定作为Meliniomyces variabilis，并且多次从邻近的杜鹃花和外生菌根根中获得具有相同ISSR谱的分离株。然而，基因体尺寸较小（< 13 厘米），并且在单个土壤核心中发现了多个基因体。遗传相关性与所研究的尺度（< 43 m）的空间分离无关，并且相距 20 km 的地点的 M. variabilis 种群在遗传上无法区分。我们得出的结论是，M. variabilis 的单个基因可以同时定殖在欧洲赤松和越橘根部，但没有证据表明形成了大型菌丝体网络。我们的数据还表明，这种普遍存在的根相关真菌的广泛分离的种群之间存在显着的基因型重叠。
P>Fungi in the Rhizoscyphus ericae aggregate have been recovered from the roots of co-occurring ericaceous shrubs and ectomycorrhizal trees. However, to date, there is no evidence that the same individual genotypes colonize both hosts, and no information on the extent of the mycelial networks that might form.Using spatially explicit core sampling, we isolated fungi from neighbouring Pinus sylvestris (ectomycorrhizal) and Vaccinium vitis-idaea (ericoid mycorrhizal) roots and applied intersimple sequence repeat (ISSR) typing to assess the occurrence and extent of shared genets.Most isolates were identified as Meliniomyces variabilis, and isolates with identical ISSR profiles were obtained from neighbouring ericoid and ectomycorrhizal roots on a number of occasions. However, genet sizes were small (< 13 cm), and several genets were found in a single soil core. Genetic relatedness was independent of spatial separation at the scales investigated (< 43 m) and M. variabilis populations from sites 20 km apart were genetically indistinguishable.We conclude that individual genets of M. variabilis can simultaneously colonize Scots pine and Vaccinium roots, but there is no evidence for the formation of large mycelial networks. Our data also suggest significant genotypic overlap between widely separated populations of this ubiquitous root-associated fungus.