Projection from a single NUCB2/nesfatin-1 neuron in the paraventricular nucleus to different brain regions involved in feeding
Projection from a single NUCB2/nesfatin-1 neuron in the paraventricular nucleus to different brain regions involved in feeding
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从室旁核中的单个 NUCB2/nesfatin-1 神经元投射到参与进食的不同大脑区域
DOI：
10.1007/s00429-015-1150-4
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发表时间：
2015
影响因子：
3.1
通讯作者：
Shimomura K.
中科院分区：
文献类型：
--
作者：
Majima Y;Kumamoto K;Takenoshita S;Shimomura K.
含有厌氧神经肽NEFA/nucleobindin 2 (NUCB2)/nesfatin-1的神经元分布在参与摄食调节的大脑区域，包括下丘脑室旁核（PVN）。在功能上，PVN中的NUCB2/nesfatin-1神经元通过下丘脑和脑干调节摄食。然而，PVN NUCB2/nesfatin-1神经元的神经网络尚未阐明。轴突侧支允许单个神经元靶向多个神经元。在某些情况下，每个目标神经元可以位于不同的细胞核中。在这里，我们发现PVN中的单个神经元向迷走背复合体（DVC）和弓形核（ARC）投射轴突侧枝，这是大脑中重要的进食调节区域。在本研究中，在DVC和ARC注射不同的逆行示踪剂后，在同一PVN神经元中检测到两种示踪剂标记的神经元，表明单个PVN神经元向DVC和ARC的轴突侧支投射。此外，免疫组织化学分析显示，从PVN到DVC和ARC的轴突侧支突出的神经元中约有50%是NUCB2/nesfatin-1神经元。我们的数据表明，PVN中的单个NUCB2/nesfatin-1神经元通过轴突侧支投射同时投射到ARC和DVC。尽管其生理意义仍有待阐明，但我们的数据为NUCB2/nesfatin-1在神经网络水平上的功能和食物摄入调节提供了新的视角。
The anorexigenic neuropeptide NEFA/nucleobindin 2 (NUCB2)/nesfatin-1-containing neurons are distributed in the brain regions involved in feeding regulation, including the hypothalamic paraventricular nucleus (PVN). Functionally, NUCB2/nesfatin-1 neurons in the PVN regulate feeding through the hypothalamus and brain stem. However, the neural network of PVN NUCB2/nesfatin-1 neurons has yet to be elucidated. Axon collateral branches allow individual neurons to target multiple neurons. In some cases, each target neuron can be located in different nuclei. Here we show that a single neuron in the PVN projects axonal collaterals to both the dorsal vagal complex (DVC) and the arcuate nucleus (ARC), which are important brain regions for feeding regulation. In this study, after injection of different retrograde tracers into the DVC and ARC, both tracer-labeled neurons were detected in the identical PVN neuron, indicating the axon collateral projections from the single PVN neuron to the DVC and ARC. Furthermore, immunohistochemical analysis revealed that approximately 50 % of the neurons with axon collateral projections from the PVN to the DVC and ARC were found to be NUCB2/nesfatin-1 neurons. Our data suggest that a single NUCB2/nesfatin-1 neuron in the PVN projects to both the ARC and the DVC with axon collateral projection. Although the physiological significance remains to be elucidated, our data offer new perspectives on NUCB2/nesfatin-1 function at the neural network level and food intake regulation.