Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia.
Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia.
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幼猪甲状腺滤泡上皮的分离和培养。
DOI：
10.1007/978-1-4939-8600-2_2
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fong,Peying
中科院分区：
文献类型：
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作者：
Lillich,JamesD;Fong,Peying
上皮组织由它们的极性和它们定向运输的能力来定义。甲状腺是一种由功能性上皮单位组成的组织，这些功能性上皮单位被组织成封闭的滤泡，其腔室由甲状腺细胞顶端限定。因此，甲状腺上皮细胞的天然排列限制了对滤泡空间的可及性，在研究跨上皮运动方面提出了挑战。这种局限性可以通过研究二维培养的甲状腺细胞来克服。在此，我们提出了从幼猪中分离甲状腺滤泡和制备高抗性极化培养物的方法。
Epithelial tissues are defined by their polarity and their ability to transport directionally. Thyroid is a tissue comprising functional epithelial units organized as enclosed follicles, with their luminal spaces defined by thyrocyte apices. Thus, the native arrangement of thyroid epithelia limits accessibility to the follicular space, presenting a challenge in studying transepithelial movements. This limitation can be overcome by studying thyrocytes grown as two-dimensional cultures. Herein we present methods for isolation of thyroid follicles from juvenile pigs and preparation of high-resistance, polarized cultures.