Biodegradation of nitrobenzene in a lysogeny broth medium by a novel halophilic bacterium Bacillus licheniformis.
Biodegradation of nitrobenzene in a lysogeny broth medium by a novel halophilic bacterium Bacillus licheniformis.
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DOI：
10.1016/j.marpolbul.2014.09.028
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发表时间：
2014-12
影响因子：
5.8
通讯作者：
T. Li;Xinping Deng;Jin-jun Wang;Yucheng Chen;Lin He;Yuchuan Sun;Caixia Song;Zhi-Feng Zhou
中科院分区：
文献类型：
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作者：
T. Li;Xinping Deng;Jin-jun Wang;Yucheng Chen;Lin He;Yuchuan Sun;Caixia Song;Zhi-Feng Zhou
地衣芽孢杆菌（Bacillus licheniformis）菌株YX 2是从农药厂活性污泥中分离到的一株能降解硝基苯的嗜盐菌。菌株YX 2可耐受高酸性和碱性条件以及高温。在溶原肉汤培养基（pH 4-9）中，YX 2对硝基苯（200 mg L-1）的降解在72 h后超过70%。在最佳降解条件下（33 °C，LB培养基中pH 7），YX 2分别在36、36、72和156 h内降解50、100、200和600 mg L− 1硝基苯。菌株YX 2在苯、苯酚和苯胺存在下也能有效降解硝基苯。此外，菌株YX 2在7%NaCl（w/w）中完全降解600 mg L− 1硝基苯。因此，我们的数据表明，菌株YX 2可能有希望从高盐度和可变pH的复杂废水中去除硝基苯。
TheBacillus licheniformisstrain YX2, a novel nitrobenzene-degrading halophilic bacterium, was isolated from active sludge obtained from a pesticide factory. Strain YX2 can withstand highly acidic and alkaline conditions and high temperatures. Degradation of nitrobenzene (200 mg L−1) by YX2 exceeded 70% after 72 h in lysogeny broth medium (pH 4–9). Under optimal degradation conditions (33 °C, pH 7 in LB medium) YX2 degraded 50, 100, 200, and 600 mg L−1nitrobenzene within 36, 36, 72, and 156 h, respectively. Even in the presence of benzene, phenol or aniline, strain YX2 efficiently degraded nitrobenzene. Furthermore, strain YX2 completely degraded 600 mg L−1nitrobenzene in 7% NaCl (w/w). Thus, our data show that strain YX2 may have promise for removing nitrobenzene from complex wastewaters with high salinity and variable pH.