Disappearance of statin, a protein marker for non-proliferating and senescent cells, following serum-stimulated cell cycle entry.
Disappearance of statin, a protein marker for non-proliferating and senescent cells, following serum-stimulated cell cycle entry.
复制标题
在血清刺激的细胞周期进入后，他汀类药物（一种非增殖和衰老细胞的蛋白质标记物）消失。
DOI：
10.1016/0014-4827(86)90211-9
复制
发表时间：
1986
影响因子：
3.7
通讯作者：
Lin,SL
中科院分区：
文献类型：
--
作者：
Wang,E;Lin,SL
他汀是一种57000 D的蛋白质，存在于静止或衰老成纤维细胞的细胞核中（Wang，E，J cell biol 100（1985）545）[1]，但在其年轻的复制对应物中不存在。多种组织的免疫组织化学研究表明，他汀类药物的存在是不再参与增殖的细胞（即终末分化的细胞）的标志物。通过免疫荧光显微镜检查血清饥饿培养物中他汀类药物的表达，通过将血清浓度从0.5%提高到10%重新启动血清饥饿培养物的复制。在加入血清之前，超过85%的细胞对他汀类药物染色呈阳性。血清刺激后12-14 h内，statin的表达迅速消失。另一方面，DNA合成水平的增加，标志着进入S期，最初观察到血清刺激后18小时，并在6小时后达到最高水平。从血清刺激后不同时间收集的细胞中获得的他汀类药物的免疫沉淀显示，他汀类药物合成水平在4 h时降低，在8 h时几乎检测不到。这些结果表明：（1）他汀类药物的合成主要发生在细胞处于静止状态时，当细胞被诱导增殖时，他汀类药物的合成迅速下降;（2）这种下降发生在从G1期向S期转变之前。
Statin, a protein of 57000 D, is present in the nuclei of quiescent or senescent fibroblasts (Wang, E, J cell biol 100 (1985) 545) [1], but is absent in their young replicating counterparts. Immunohistochemical survey of a variety of tissues demonstrates that the presence of statin is a marker for cells that are no longer involved in proliferation, i.e. those cells that are terminally differentiated. Statin expression was examined by immunofluorescence microscopy in serum-starved cultures whose replication had been reinitiated by raising the serum concentration from 0.5 to 10%. Prior to serum addition, more than 85% of the cells stained positively for statin. After stimulation with serum, the expression of statin disappeared rapidly within the first 12–14 h. On the other hand, an increase in the level of DNA synthesis, signifying entry into S phase, was observed initially at 18 h after serum stimulation, and reached maximal levels 6 h later. Immunoprecipitation of statin derived from cells harvested at different intervals after serum stimulation revealed that the level of statin synthesis was reduced by 4 h and was hardly detectable at 8 h. These results demonstrate that (1) the synthesis of statin occurs primarily when cells are in a quiescent state, and declines rapidly when cells are induced to proliferate; (2) this decline precedes the transition from G1 to S phase.