Evidence for frequent recombination within species Human enterovirus B based on complete genomic sequences of all thirty-seven serotypes
Evidence for frequent recombination within species Human enterovirus B based on complete genomic sequences of all thirty-seven serotypes
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DOI：
10.1128/jvi.78.2.855-867.2004
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发表时间：
2004-01-01
影响因子：
5.4
通讯作者：
Pallansch，MA
中科院分区：
文献类型：
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作者：
Oberste，MS;Maher，K;Pallansch，MA
人肠道病毒B（HEV-B）属角状病毒科，包括柯萨奇病毒A9；柯萨奇病毒B1至B6；埃可病毒1 ~ 7、9、11 ~ 21、24 ~ 27和29 ~ 33；肠病毒69和73。我们确定了未在公共数据库中显示的其余22种HEV-B血清型的完整基因组序列，并将这些序列与GenBank中先前可用的完整序列相结合进行了分析。在基因组的所有区域，HEV-B成员相对于所有其他人类肠道病毒物种是单系的，除了在5 ' -非翻译区（NTR），在那里它们已知与HEV-A成员聚集。在HEV-B中，由基因组的结构区（P1）和非结构区（P2和P3）构建的系统发育不一致，表明发生了重组。整个基因组的相似性图和引导扫描分析确定了给定菌株序列的系统发育发生变化的多个位点，表明了潜在的重组点。这些点分布在5 ' -NTR和整个P2和P3中，但在衣壳内没有发现> - 80% bootstrap支持的位点。个体序列比较和系统发育分析表明，HEV-B成员在多个场合相互重组，导致基因组非结构区域中来自多个亲本病毒的复杂序列镶嵌。我们得出结论，RNA重组是肠病毒进化的一种常见机制，并且在基因组的非结构区域（P2和P3）内的重组仅在同一物种的成员中被观察到。
The speciesHuman enterovirus B(HEV-B) in the familyPicornaviridaeconsists of coxsackievirus A9; coxsackieviruses B1 to B6; echoviruses 1 to 7, 9, 11 to 21, 24 to 27, and 29 to 33; and enteroviruses 69 and 73. We have determined complete genome sequences for the remaining 22 HEV-B serotypes whose sequences were not represented in public databases and analyzed these in conjunction with previously available complete sequences in GenBank. Members of HEV-B were monophyletic relative to all other human enterovirus species in all regions of the genome except in the 5′-nontranslated region (NTR), where they are known to cluster with members of HEV-A. Within HEV-B, phylogenies constructed from the structural (P1) and nonstructural regions of the genome (P2 and P3) are incongruent, suggesting that recombination had occurred. Similarity plots and bootscanning analysis across the complete genome identified multiple sites at which the phylogeny of a given strain's sequence shifted, indicating potential recombination points. These points are distributed in the 5′-NTR and throughout P2 and P3, but no sites with >80% bootstrap support were identified within the capsid. Individual sequence comparisons and phylogenetic analyses suggest that members of HEV-B have recombined with one another on multiple occasions, resulting in a complex mosaic of sequences derived from multiple parental viruses in the nonstructural regions of the genome. We conclude that RNA recombination is a common mechanism for enterovirus evolution and that recombination within the nonstructural regions of the genome (P2 and P3) has been observed only among members of the same species.