Effects of Quercetin Metabolites on Triglyceride Metabolism of 3T3-L1 Preadipocytes and Mature Adipocytes
Effects of Quercetin Metabolites on Triglyceride Metabolism of 3T3-L1 Preadipocytes and Mature Adipocytes
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DOI：
10.3390/ijms20020264
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发表时间：
2019-01-02
影响因子：
5.6
通讯作者：
Portillo, Maria P.
中科院分区：
文献类型：
--
作者：
Eseberri, Itziar;Miranda, Jonatan;Portillo, Maria P.
槲皮素(Q)代谢迅速，其代谢产物的潜在活性值得关注。我们的目的是评估Q代谢物在成熟和前期脂肪细胞中降低甘油三酯的作用，并与Q诱导的效果进行比较。3T3-L1成熟和前期脂肪细胞分别用0.1、1和10 μ M的Q、tamarixetin （TAM）、异鼠李素（ISO）、槲皮素-3- o -葡萄糖醛酸酯（3G）、槲皮素-3- o -硫酸盐（3S）以及3S和槲皮素-4- o -硫酸盐（4S）混合物（3S+4S）处理。采用分光光度法测定两种细胞类型的甘油三酯（TG）含量，以及成熟脂肪细胞培养液中的游离脂肪酸（FFA）和甘油含量。RT-PCR检测基因表达。在成熟脂肪细胞中，Q降低了1 μ M和10 μ M时的TG， 1 μ M和10 μ M时的3S代谢物，以及10 μ M时3S+4S混合物。3S处理改变了葡萄糖摄取和TG组装，但没有改变脂肪分解或凋亡。在分化过程中，只有10 μ M的ISO降低了TG含量，生理剂量的Q也降低了TG含量。综上所述，3S代谢物参与了Q的体内清除作用，而ISO、3G、4S和TAM代谢物没有参与。
Quercetin (Q) has rapid metabolism, which may make it worthwhile to focus on the potential activity of its metabolites. Our aim was to evaluate the triglyceride-lowering effects of Q metabolites in mature and pre-adipocytes, and to compare them to those induced by Q. 3T3-L1 mature and pre-adipocytes were treated with 0.1, 1 and 10 mu M of Q, tamarixetin (TAM), isorhamnetin (ISO), quercetin-3-O-glucuronide (3G), quercetin-3-O-sulfate (3S), as well as with 3S and quercetin-4-O-sulfate (4S) mixture (3S+4S). Triglyceride (TG) content in both cell types, as well as free fatty acid (FFA) and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene expression was assessed by RT-PCR. In mature adipocytes, Q decreased TG at 1 and 10 mu M, 3S metabolite at 1 and 10 mu M, and 3S+4S mixture at 10 mu M. 3S treatment modified the glucose uptake, and TG assembling, but not lipolysis or apoptosis. During differentiation, only 10 mu M of ISO reduced TG content, as did Q at physiological doses. In conclusion, 3S metabolite but not ISO, 3G, 4S and TAM metabolites can contribute to the in vivo delipidating effect of Q.