Increase of proliferating oligodendroglial progenitors in the adult mouse brain upon Sonic hedgehog delivery in the lateral ventricle
Increase of proliferating oligodendroglial progenitors in the adult mouse brain upon Sonic hedgehog delivery in the lateral ventricle
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DOI：
10.1111/j.1471-4159.2006.03896.x
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发表时间：
2006-07-01
影响因子：
4.7
通讯作者：
Traiffort, Elisabeth
中科院分区：
文献类型：
--
作者：
Loulier, Karine;Ruat, Martial;Traiffort, Elisabeth
声波刺猬信号是维持出生后脑室下区干细胞生态位和成熟海马神经祖细胞增殖所必需的。我们在此表明，将音刺猬蛋白递送至成年小鼠的侧脑室可增加胼胝体和大脑皮层的细胞增殖。在后一个区域中，注射 2 天后表达蛋白聚糖 NG2 的神经祖细胞数量增加。在这两个区域中，在表达少突胶质细胞转录因子 Olig1 的细胞中观察到转录靶基因 Patched 的 mRNA 上调。腺病毒介导的 Sonic Hedgehog 进入侧脑室 26 天后，大脑皮层和胼胝体中新生成的细胞受到少突胶质细胞生成初始步骤的影响，表达少突胶质细胞标记物 DM20 的细胞数量增加了 50%。我们的实验表明，在传递 Sonic humig 蛋白后，大脑皮层和胼胝体中少突胶质细胞前体细胞的数量可以增加，并强调了成人大脑动员髓鞘形成前细胞池的潜在能力。
Sonic hedgehog signaling is required for the maintenance of stem cell niches in the postnatal subventricular zone and the proliferation of neural progenitors in the mature hippocampus. We show here that delivery of Sonic hedgehog protein into the lateral ventricle of adult mice increases cell proliferation in the corpus callosum and cerebral cortex. In this latter area, the number of neural progenitors expressing the proteoglycan NG2 is enhanced 2 days after the injection. In both areas, mRNA up-regulation of the transcriptional target gene Patched was observed in cells expressing the oligodendroglial transcription factor Olig1. Twenty-six days following the adenovirus-mediated delivery of Sonic hedgehog into the lateral ventricle, newly generated cells in the cerebral cortex and in the corpus callosum are influenced towards the initial steps of oligodendrogenesis, as indicated by a 50% increase in the number of cells expressing the oligodendroglial marker DM20. Our experiments demonstrate that the number of oligodendrocyte precursor cells in the cerebral cortex and corpus callosum can be increased upon delivery of Sonic hedgehog proteins and highlight the potential capacity of the adult brain to mobilize a pool of premyelinating cells.