Chromosomal rearrangements formed by rrn recombination do not improve replichore balance in host-specific Salmonella enterica serovars.
Chromosomal rearrangements formed by rrn recombination do not improve replichore balance in host-specific Salmonella enterica serovars.
复制标题
DOI：
10.1371/journal.pone.0013503
复制
发表时间：
2010-10-19
期刊：
影响因子：
3.7
通讯作者：
Maloy S
中科院分区：
文献类型：
--
作者：
Matthews TD;Edwards R;Maloy S
肠炎沙门氏菌的∼2,600个血清型中，大多数具有广泛的宿主范围和保守的基因序列。相反，一些沙门氏菌血清型是宿主特有的，经常表现出由于RRN操纵子之间的重组而产生的大的染色体重排。一种解释这些重排的假说认为，由于致病岛和前驱噬菌体的水平转移而引入的复制中心不平衡推动了染色体重排，试图改善平衡。通过将自然产生的染色体排列类型与理论上可能的排列类型进行比较，并使用计算器估计它们的复制体平衡，直接检验了这一假设。除已鉴定的属于宿主特异性血清型的菌株外，还确定了22个血清型的鸡冠状芽胞杆菌的排列类型。在212株寄主特异性菌株中，1440种可能的排列类型中只有48种被鉴定出来。虽然大多数自然发生的排列类型的复制库是平衡的，但大多数理论上的排列类型的复制库是不平衡的。此外，最常见的重排类型不会改变复制核平衡。结果并不支持复制核不平衡导致这些重排的假设，并表明这些重排可以由宿主特有的生活方式的一些方面来解释。
Most of the ∼2,600 serovars of Salmonella enterica have a broad host range as well as a conserved gene order. In contrast, some Salmonella serovars are host-specific and frequently exhibit large chromosomal rearrangements from recombination between rrn operons. One hypothesis explaining these rearrangements suggests that replichore imbalance introduced from horizontal transfer of pathogenicity islands and prophages drives chromosomal rearrangements in an attempt to improve balance. This hypothesis was directly tested by comparing the naturally-occurring chromosomal arrangement types to the theoretically possible arrangement types, and estimating their replichore balance using a calculator. In addition to previously characterized strains belonging to host-specific serovars, the arrangement types of 22 serovar Gallinarum strains was also determined. Only 48 out of 1,440 possible arrangement types were identified in 212 host-specific strains. While the replichores of most naturally-occurring arrangement types were well-balanced, most theoretical arrangement types had imbalanced replichores. Furthermore, the most common types of rearrangements did not change replichore balance. The results did not support the hypothesis that replichore imbalance causes these rearrangements, and suggest that the rearrangements could be explained by aspects of a host-specific lifestyle.
登录
查看更多内容
影响因子：
4.4
作者：
Gallois, A;Klein, JR;Nauseef, WM
通讯作者：
Nauseef, WM
影响因子：
3.2
作者：
Alokam, S;Liu, SL;Sanderson, KE
通讯作者：
Sanderson, KE
影响因子：
4.5
作者：
Esnault E;Valens M;Espéli O;Boccard F
通讯作者：
Boccard F
影响因子：
4.4
作者：
Helm, RA;Maloy, S
通讯作者：
Maloy, S
影响因子：
5.6
作者：
Duggin, Iain G.;Bell, Stephen D.
通讯作者：
Bell, Stephen D.