Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis
Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis
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DOI：
10.1016/j.plaphy.2013.05.025
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发表时间：
2013-09-01
影响因子：
6.5
通讯作者：
Liu, Gongshe
中科院分区：
文献类型：
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作者：
Cheng, Liqin;Li, Xiaoxia;Liu, Gongshe
羊草[Leymus chinensis（Trin.）Tzvel.]是一个占主导地位的，根茎草，具有广泛的可塑性，在适应各种恶劣的环境。基于454个盐胁迫下的高通量测序（GS FIX）数据，从绵羊草中鉴定出一个功能未知的MYB相关基因LcMYB 1。组织特异性表达谱显示LcMYB 1基因在不同组织中普遍表达，在根茎和穗中观察到较高的表达水平。高盐、干旱和脱落酸对LcMYB 1的表达有明显的诱导作用，低温对LcMYB 1的表达有轻微的诱导作用。LcMYB 1与绿色荧光蛋白（GFP）的融合蛋白定位于细胞核，酵母单杂交分析表明LcMYB 1是转录活性的激活因子。LcMYB1过表达的植物比野生型植物更耐盐胁迫。盐胁迫条件下，转基因拟南芥中脯氨酸和可溶性糖的含量高于野生型。LcMYB1的过表达增强了P5CS1的表达水平，并抑制了其他盐胁迫反应基因标记。这些发现表明，LcMYB1影响复杂的盐胁迫响应信号网络，通过促进不同的途径比经典的DREB1A和MYB2介导的信号通路。此外，LcMYB 1是一个很有前途的基因资源，用于提高作物的耐盐性。(c)2013年Elsevier Masson SAS。All rights reserved.
Sheepgrass [Leymus chinensis (Trin.) Tzvel.] is a dominant, rhizomatous grass that has extensive plasticity in adapting to various harsh environments. Based on data from 454 high-throughput sequencing (GS FIX) exposure to salt stress, an unknown functional MYB-related gene LcMYB1 was identified from sheepgrass. Tissue specific expression profiles showed that the LcMYB1 gene was expressed ubiquitously in different tissues, with higher expression levels observed in the rhizome and panicle. The expression of LcMYB1 was induced obviously by high salt, drought and abscisic acid and was induced slightly by cold. A fusion protein of LcMYB1 with green fluorescent protein (GFP) was localized to the nucleus, and yeast one-hybrid analysis indicated that LcMYB1 was an activator of transcriptional activity. LcMYB1-overexpressing plants were more tolerant to salt stress than WT plants. The amounts of proline and soluble sugars were higher in transgenic Arabidopsis than in WT plants under salt stress conditions. The overexpression of LcMYB1 enhanced the expression levels of P5CS1 and inhibited other salt stress response gene markers. These findings demonstrate that LcMYB1 influences the intricate salt stress response signaling networks by promoting different pathways than the classical DREB1A- and MYB2-mediated signaling pathway. Additionally, LcMYB1 is a promising gene resource for improving salinity tolerance in crops. (c) 2013 Elsevier Masson SAS. All rights reserved.