Photonic crystal microcavity sensor for ultracompact monitoring of reaction kinetics and protein concentration
Photonic crystal microcavity sensor for ultracompact monitoring of reaction kinetics and protein concentration
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DOI：
10.1016/j.snb.2009.06.007
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发表时间：
2009-08-18
影响因子：
8.4
通讯作者：
Grot, Annette
中科院分区：
文献类型：
--
作者：
Zlatanovic, Sanja;Mirkarimi, Laura W.;Grot, Annette
我们报告了使用总面积为 50 μ m(2) 且有效检测面积为 0.272 μ m(2) 的光子晶体微腔传感器对生理缓冲液中蛋白质结合进行时间分辨无标记监测。我们使用这种超紧凑传感器来监测抗生物素与生物素化牛血清白蛋白 (b-BSA) 的结合，并测量 6.94 x 10(7) M(-1) 的亲和常数。我们证明这种光子晶体传感器可用于浓度范围从皮摩尔到微摩尔的抗生物素检测。抗生物素的检测下限低于 20 pM。相当于传感器表面上的结合材料少于 4.5 fg，微腔模态体积中的分子少于 80 个。该传感器还能够测量小分子物质的结合，例如芳环 (98 Da)。此外，我们表明传感器的活性表面可以成功再生并在后续的蛋白质结合实验中重复使用。给出了实验数据和理论数据的比较，并讨论了传感器当前在噪声方面的实验局限性。 (C) 2009 Elsevier B.V. 保留所有权利。
We report on time-resolved label-free monitoring of protein binding in a physiological buffer using a photonic crystal microcavity sensor of total area 50 mu m(2) with an effective detection area of 0.272 mu m(2). We use this ultracompact sensor to monitor the binding of anti-biotin to biotinylated-bovine serum albumin (b-BSA), and measure an affinity constant of 6.94 x 10(7) M(-1). We show that this photonic crystal sensor can be used for anti-biotin detection at concentrations ranging from picomolar to micromolar. The lower limit of detection for anti-biotin is less than 20 pM. corresponding to less than 4.5 fg of bound material on the sensor surface and fewer than 80 molecules in the modal volume of the microcavity. The sensor also has the capability of measuring binding of small molecular species such as aromatic rings (98 Da). Furthermore, we show that the active surface of the sensor can be successfully regenerated and re-used in subsequent protein binding experiments. A comparison of experimental and theoretical data is given, and the current experimental limitations of the sensor with regard to noise are discussed. (C) 2009 Elsevier B.V. All rights reserved.