EXPRESSED SEQUENCE TAGS (EST) IDENTIFY GENES PREFERENTIALLY EXPRESSED IN CATFISH CHEMOSENSORY TISSUES
EXPRESSED SEQUENCE TAGS (EST) IDENTIFY GENES PREFERENTIALLY EXPRESSED IN CATFISH CHEMOSENSORY TISSUES
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DOI：
10.1016/0169-328x(94)90237-2
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发表时间：
1994-06-01
期刊：
影响因子：
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通讯作者：
MARGOLIS, FL
中科院分区：
文献类型：
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作者：
BETTINI, E;PORTA, AR;MARGOLIS, FL
表达序列标签（EST）的鲶鱼（癍点叉尾鱼）进行了鉴定和特点的鸟枪测序耦合北方分析。我们已经确定和表征了一些cDNA克隆鲶鱼嗅觉粘膜库，显示差异组织表达，包括几个丰富的化学感觉组织。在研究的新的cDNA克隆是嗅觉特异性β-微管蛋白和钙结合蛋白的S-100家族的一个新成员，该家族在触须，嗅粘膜和鳃中高度表达，但不在大脑中。还鉴定了几个低丰度mRNA的克隆，包括一个表现出许多转录因子典型的碱性-heliu-环-螺旋（b-HLH）基序的克隆。还获得了其mRNA差异表达但功能未知的其他cDNA克隆。这些结果表明，可以很容易地鉴定出在化学感受组织中富集的新基因产物。
Expressed sequence tags (ESTs) for the catfish (Ictalurus punctatus) were identified and characterized by shotgun sequencing coupled to Northern analysis. We have identified and characterized a number of cDNA clones from a catfish olfactory mucosal library that show differential tissue expression including several that are enriched in chemosensory tissue. Among the novel cDNA clones studied were an olfactory specific beta-tubulin and a novel member of the S-100 family of calcium-binding proteins that is highly expressed in barbel, olfactory mucosa and gill, but not in brain. Several clones of low abundance mRNAs were also identified, including one manifesting a basic-heliu-loop-helix (b-HLH) motif that is typical of many transcription factors. Additional cDNA clones whose mRNAs are differentially expressed, but are of unknown function, were also obtained. These results demonstrate the ease with which novel gene products enriched in chemosensory tissues can be identified.