Experimental simulation of transmission of an obligate aphid pathogen with aphid flight dispersal
Experimental simulation of transmission of an obligate aphid pathogen with aphid flight dispersal
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通过蚜虫飞行传播专性蚜虫病原体传播的实验模拟。
DOI：
10.1111/j.1462-2920.2005.00869.x
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Feng, MG
中科院分区：
文献类型：
--
作者：
Chen, C;Feng, MG
广泛传播的虫霉引起的真菌病，通常调节蚜虫种群是最有可能与感染的翅类飞行。通过模拟飞行和飞行后殖民的Myzus periscae alates暴露于Pandora neoaphidis，一种常见的专性蚜虫病原体流行的世界各地的孢子阵雨，这一假设进行了检查。总共有407只有翅类动物被分批次进行淋浴，然后在计算机监控的飞行磨坊系统中单独飞行，并在20-23摄氏度下在卷心菜叶子上饲养14天的自由繁殖和后代群体内的传染性感染。80.6%的菌株平均飞行2.6km，1-5 h，存活3.2d，产毒5.3g，并成功地将其感染传给后代。然而，9.8%的飞行翅类没有留下后代，虽然他们生存至少1天前真菌感染，而其余的没有真菌感染，产生显着更多的真菌在第一周。感染或未感染的有翅类的飞行距离（0.01- 10.2km）和飞行时间（0.1- 8.3km）均与飞行距离呈指数相关（r（2）≥ 0.98）。按飞行距离<1.0，1.0-3.0，3.0-5.0和>5.0 km分组，存活蚜虫数和真菌化个体数比例随定殖天数的变化分别符合复logistic模型（r（2）= 0.984）和修正Gompertz模型（r（2）= 0.978）。两种模型均以飞行距离、飞行后存活时间、真菌病前期繁殖力和初次感染率为自变量来影响发育率。这些结果突出了感染的有翅类在蚜虫中广泛传播的新蚜虫疫霉引起的真菌病中的重要作用。
The wide dispersal of Entomophthorales-caused mycoses that usually regulate aphid populations is most likely to be associated with the flight of infected alates. This hypothesis was examined via simulated flight and postflight colonization of Myzus periscae alates exposed to spore showers of Pandora neoaphidis, a common obligate aphid pathogen prevalent world wide. A total number of 407 alates were showered in different batches, then individually flown in a computer-monitoring flight mill system and reared on cabbage leaves for 14-day free reproduction and contagious infection within progeny colonies at 20-23 degrees C. On average, 80.6% of them flew 2.6 km in 1-5 h, survived 3.2 days, produced 5.3 nymphs, and transmitted their infection to progeny successfully. However, 9.8% of the flown alates left no progeny although they survived at least 1 day prior to mycosis while the rest were not mycosed, producing significantly more nymphs during the first week. The flight distances of the infected (0.01-10.2 km) or uninfected alates (0.1-8.3 km) were exponentially correlated to the flight time (r( 2) >or= 0.98). When grouped by the flight ranges of <1.0, 1.0-3.0, 3.0-5.0 and >5.0 km, the number of live aphids and the proportion of mycosed individuals per progeny colony over colonization days fit well to a complex logistic model (r( 2) = 0.984) and modified Gompertz model (r( 2) = 0.978) respectively. Both models included flight distance, postflight survival time, premycosis fecundity and primary infection rate as independent variables to affect the developmental rates. The results highlight the significant role of infected alates in the wide dispersal of P. neoaphidis-caused mycoses among aphids.