Immunohistochemical expression of matrix metalloproteinases in photodamaged skin by photodynamic therapy
Immunohistochemical expression of matrix metalloproteinases in photodamaged skin by photodynamic therapy
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DOI：
10.1111/j.1365-2133.2009.09326.x
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发表时间：
2009-09-01
影响因子：
10.3
通讯作者：
Manela-Azulay, M.
中科院分区：
文献类型：
--
作者：
Issa, M. C. Almeida;Pineiro-Maceira, J.;Manela-Azulay, M.
背景光动力疗法（PDT）已被描述用于光老化治疗，但其作用机制尚不清楚。虽然PDT诱导的基质金属蛋白酶（MMP）的表达和胶原蛋白的产生已在正常皮肤和炎症性疾病中进行了研究，有没有关于PDT的光损伤skin. ObjectiveTo评估光损伤皮肤的细胞外基质的影响的报告氨基乙酰丙酸甲酯（MAL）-PDT诱导的皮肤重塑的组织学和免疫组化研究。光源为发光二极管（635 nm，37 J cm（-2））。所有患者在治疗前和治疗后3个月和6个月进行皮肤活检。免疫组化检测I型和III型胶原、MMP-1、MMP-3、MMP-7、MMP-9、MMP-12和金属蛋白酶组织抑制剂-1。治疗后3个月，真皮层MMP-9的表达明显增加（P = 0.002）。治疗后3个月（P = 0 0 0 2）和6个月（P = 0 0 0 1），Ⅰ型胶原蛋白表达明显增加。两个疗程的MAL-PDT在治疗后3个月增加了真皮中MMP-9和I型胶原的免疫组织化学表达。
Background Photodynamic therapy (PDT) has been described for photoageing treatment, but its mechanism of action is not clarified. Although PDT-induced matrix metalloproteinase (MMP) expression and collagen production have been studied in normal skin and in inflammatory disease, there is no report about the effect of PDT on the extracellular matrix in photodamaged skin.Objectives To evaluate skin remodelling induced by methyl aminolaevulinate (MAL)-PDT in photodamaged skin by histological and immunohistochemical studies.Methods Fourteen patients were treated with two sessions of MAL-PDT. The light source was a light-emitting diode (635 nm, 37 J cm(-2)). Skin biopsies were performed in all patients before and at 3 and 6 months after treatment. Immunohistochemical studies evaluated collagen types I and III, MMP-1, MMP-3, MMP-7, MMP-9, MMP-12 and tissue inhibitor of metalloproteinases-1.Results Global improvement in photodamaged skin was observed. A significant increase in expression of MMP-9 in the dermis was detected at 3 months after treatment (P = 0 002). Significant increases in the expression of collagen type I at 3 months (P = 0 002) and at 6 months after treatment (P = 0 001) were also observed.Conclusions Skin remodelling induced by MAL-PDT was demonstrated in photodamaged skin. Two sessions of MAL-PDT increases immunohistochemical expression of MMP-9 in the dermis at 3 months after treatment, and also of collagen type I.