Comparison of QTLs for early elongation ability between two floating rice cultivars with a different phylogenetic origin
Comparison of QTLs for early elongation ability between two floating rice cultivars with a different phylogenetic origin
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DOI：
10.1270/jsbbs.55.1
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发表时间：
2005-03-01
期刊：
影响因子：
2.4
通讯作者：
Nemoto, K
中科院分区：
文献类型：
--
作者：
Tang, DQ;Kasai, Y;Nemoto, K
在植物生长早期开始茎伸长的能力是漂浮水稻在洪水存在下的主要生存机制。我们之前利用一个Patnai23（非浮动品种）x Goai（浮动品种）F-2群体，在3号染色体和12号染色体上发现了两个与这种“早期伸长能力”相关的数量性状位点（qtl）。本研究的目的是：(1)鉴定Habiganj Aman VIII（一个浮动品种）与Patnai23杂交的早期伸长能力qtl；(2)证实我们之前的双列分析结果，即Habiganj Aman VIII可能比Goai含有更多的早期伸长能力基因。利用85个（9个RFLP和76个SSR）标记对192个F2植株进行了基因型分析，并对其早期伸长能力进行了评价。我们根据显示伸长的最低节间的位置（“最低伸长节间”，LEI）来衡量早期伸长能力，因为LEI位置与茎伸长开始的时间平行。结果，在12号染色体上检测到的一个大QTL似乎在两个杂交中是共同的。在1号染色体上检测到一个小的QTL是Habiganj Aman VIII与Patnai23杂交所特有的，这与我们之前的双列分析结果一致。3号染色体上未发现qtl。这些结果表明：(1)从表型变异的百分比和不同背景下的一致性来看，第12染色体上的QTL可能是早期伸长能力表达的最重要的QTL；(2)双列杂交分析可能是研究不同亲本遗传控制差异的有效方法。
The ability to initiate stem elongation at the early growth stages of a plant is the major survival mechanism of floating rice in the presence of flooding. We previously identified two quantitative trait loci (QTLs) for this "early elongation ability" on chromosomes 3 and 12 using a Patnai23 (a non-floating cultivar) x Goai (a floating cultivar) F-2 population. The objectives of the present study were: (1) to identify QTLs for early elongation ability in the cross Habiganj Aman VIII (a floating cultivar) x Patnai23, and (2) to confirm the results obtained in our previous diallel analysis indicating that Habiganj Aman VIII might harbour additional gene(s) for early elongation ability compared with Goai. The genotype of a total of 192 F2 plants was investigated using 85 (9 RFLP and 76 SSR) markers and the plants were assessed for early elongation ability. We scaled early elongation ability based on the position of the lowermost internode that exhibited elongation ("lowest elongated internode", LEI), since the LEI position parallels the time of the onset of stem elongation. As a result, a large QTL detected on chromosome 12 seemed to be common to the two crosses. A small QTL detected on chromosome I was unique to the Habiganj Aman VIII x Patnai23 cross, which was consistent with the results obtained in our previous diallel analysis. No QTLs were found on chromosome 3 in this cross. These results suggested that (1) the QTL on chromosome 12 might be the most important one for the expression of early elongation ability in terms of the to percentage of phenotypic variation and consistency in different backgrounds, and (2) diallel analysis might be an effective method for examining differences in genetic control among diverse parents.