SIMILAR LETHAL EFFECT IN MAMMALIAN-CELLS FOR 2 RADIOISOTOPES OF COPPER WITH DIFFERENT DECAY SCHEMES, CU-64 AND CU-67
SIMILAR LETHAL EFFECT IN MAMMALIAN-CELLS FOR 2 RADIOISOTOPES OF COPPER WITH DIFFERENT DECAY SCHEMES, CU-64 AND CU-67
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DOI：
10.1080/09553008914550421
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发表时间：
1989-03-01
影响因子：
2.6
通讯作者：
SISSOEFF, I
中科院分区：
文献类型：
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作者：
APELGOT, S;COPPEY, J;SISSOEFF, I
掺入人恶性（A549）或猴非恶性（CVI）细胞中的64 Cu衰变导致细胞死亡。当作为生长培养基中引入的放射性（t=0时的μ Ci/ml）的函数作图时，残余集落形成能力呈指数下降。A549电池的相应曲线斜率比CVI电池的相应曲线斜率更大。不同的数据表明，细胞致死事件是64 Cu嬗变的结果，而不是同时发射的β-Cu的辐射的结果。和β +粒子持液结果表明，致命事件是不可挽回的。铜的另一种放射性同位素67 Cu的衰变导致细胞死亡，具有与64 Cu相同的指数存活曲线和相同的致死效率，尽管它们的衰变方案不同。在与64 Cu和67 Cu相同的实验条件下，两种铜同位素的致死效率接近于以碘脱氧尿苷形式使用的125 I的致死效率。放射性铜嬗变的高致死效率提出了关于已知存在于这种大分子中的微量铜原子在DNA功能中的作用的问题。放射性铜嬗变的致命后果表明，与DNA结合的铜原子对细胞功能至关重要。
The decays of 64Cu incorporated in human malignant (A549) or monkey non-malignant (CVI) cells lead to cell death. When plotted as a function of the radioactivity introduced in the growth medium (.mu.Ci/ml at t=0), the residual colony-forming capability decreases exponentially. The slope of the corresponding curve is steper for A549 than for CVI cells. Different data show that the cellular lethal event is a consequence of 64Cu transmutation and not of the irradiation by the simultaneously emitted .beta.- and .beta.+ particles. Liquid holding results show that the lethal event is irreparable. The decays of 67Cu, another radioisotope of copper, lead to cell death with the same exponential survival curve and the same lethal efficiency as for 64Cu, in spite of their different decay schemes. The lethal efficiency of both copper isotopes is close to that of 125I utilized in the form of iododeoxyuridine under the same experimental conditions as 64Cu and 67Cu. The high lethal efficiency of radioactive copper transmutations raises questions about the role in DNA functioning of copper atoms known to be present in trace amounts in this macromolecule. The lethal consequence of radioactive copper transmutations suggests that the copper atoms bound to DNA are essential for cellular functioning.