Host species and developmental stage, but not host social structure, affects bacterial community structure in socially polymorphic bees
Host species and developmental stage, but not host social structure, affects bacterial community structure in socially polymorphic bees
复制标题
DOI：
10.1111/1574-6941.12302
复制
发表时间：
2014-05-01
影响因子：
4.2
通讯作者：
Mueller, Ulrich G.
中科院分区：
文献类型：
--
作者：
McFrederick, Quinn S.;Wcislo, William T.;Mueller, Ulrich G.
社会传播和宿主发育阶段被认为深刻影响与蜜蜂和熊蜂相关的细菌群落结构，但这些想法尚未在其他蜜蜂物种中探索。嗜盐蜂Megalopta centralis和M.genalis表现出种群内社会多态性，我们利用它来测试细菌群落是否因宿主社会结构、发育阶段或宿主物种而不同。我们收集了社会和孤独的Megalopta巢和采样蜜蜂和巢内容从主机发展的各个阶段。为了调查这些细菌群落，我们使用16S rRNA基因454焦磷酸测序。我们没有发现社会结构的影响，但发现宿主物种和发育阶段的差异。沃尔巴克氏体患病率不同的两个宿主物种。与不同发育阶段相关的细菌群落似乎是由环境获得的细菌驱动的。一个kunkeei乳酸杆菌分支细菌，始终与其他蜜蜂物种是占主导地位的花粉规定和幼虫样品，但不太丰富的成熟幼虫和蛹。觅食的成年人似乎经常重新获得L.kunkeei分支细菌，可能是在花觅食。环境传播似乎是更重要的比社会传播的Megalopta蜜蜂之间的社会和孤立的行为。
Social transmission and host developmental stage are thought to profoundly affect the structure of bacterial communities associated with honey bees and bumble bees, but these ideas have not been explored in other bee species. The halictid bees Megalopta centralis and M.genalis exhibit intrapopulation social polymorphism, which we exploit to test whether bacterial communities differ by host social structure, developmental stage, or host species. We collected social and solitary Megalopta nests and sampled bees and nest contents from all stages of host development. To survey these bacterial communities, we used 16S rRNA gene 454 pyrosequencing. We found no effect of social structure, but found differences by host species and developmental stage. Wolbachia prevalence differed between the two host species. Bacterial communities associated with different developmental stages appeared to be driven by environmentally acquired bacteria. A Lactobacillus kunkeei clade bacterium that is consistently associated with other bee species was dominant in pollen provisions and larval samples, but less abundant in mature larvae and pupae. Foraging adults appeared to often reacquire L.kunkeei clade bacteria, likely while foraging at flowers. Environmental transmission appears to be more important than social transmission for Megalopta bees at the cusp between social and solitary behavior.