A NEW TECHNIQUE FOR VISUALIZATION OF THE HUMAN RETINAL VASCULATURE
A NEW TECHNIQUE FOR VISUALIZATION OF THE HUMAN RETINAL VASCULATURE
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DOI：
10.1001/archopht.1992.01080140123039
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发表时间：
1992-02-01
影响因子：
--
通讯作者：
MCLEOD, DS
中科院分区：
文献类型：
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作者：
LUTTY, GA;MCLEOD, DS
腺苷二磷酸酶是一种内皮细胞外酶，可将5'-二磷酸腺苷水解为单磷酸腺苷，已被用于组织化学研究人类视网膜血管系统的平载或可切片的平埋组织。在所有视网膜中，反应产物几乎完全局限于脉管系统，动脉比静脉或毛细血管有更多的反应产物。在内皮细胞、平滑肌细胞、周细胞和红细胞中观察到腺苷二磷酸酶的活性。在更精细的技术版本中，腺苷二磷酸酶孵育的视网膜被平埋在甲基丙烯酸乙二醇酯中。这允许在双重视角下研究视网膜血管系统：整体使用暗场显微镜和特定区域的组织学切片以进一步评估。该技术简单，并保留血管结构，因为视网膜是完整的，血管通畅可以确定。
Adenosine diphosphatase, an endothelial cell ectoenzyme that hydrolyses adenosine 5'-diphosphate to adenosine monophosphate, has been used histochemically to study the human retinal vasculature in flat mounts or as flat-embedded tissue that can be sectioned. In all retinas, reaction product was confined almost exclusively to the vasculature, with arteries having more reaction product than veins or capillaries. Adenosine diphosphatase activity was observed in endothelium, smooth-muscle cells, pericytes, and erythrocytes. In the more elaborate version of the technique, the adenosine diphosphatase-incubated retinas were flat embedded in glycol methacrylate. This allowed study of the retinal vasculature in dual perspective: en bloc with the use of dark-field microscopy and specific areas of interest histologically sectioned for further evaluation. The technique is simple and preserves angioarchitecture because the retinas are intact, and patency of the vasculature can be determined.