Fabrication of a biomimetic nanochannel logic platform and its applications in the intelligent detection of miRNA related to liver cancer
Fabrication of a biomimetic nanochannel logic platform and its applications in the intelligent detection of miRNA related to liver cancer
复制标题
仿生纳米通道逻辑平台的构建及其在肝癌相关miRNA智能检测中的应用
DOI：
10.1021/acs.analchem.0c00147
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发表时间：
2020
影响因子：
7.4
通讯作者：
Jing-Juan Xu
中科院分区：
文献类型：
--
作者：
Siqi Zhang;Jiaxi Cheng;Wei Shi;Kai-Bin Li;De-Man Han;Jing-Juan Xu
基于纳米通道的分析技术在核酸测序和生物分子的高灵敏度检测方面具有巨大的应用潜力。然而，传统的固态纳米通道传感器的灵敏度受到缺乏有效的信号放大策略的阻碍，这限制了其在分析化学领域的实用性。在这里，我们选择了与聚乙烯亚胺和Zr 4+结合的固态纳米通道作为传感平台。该传感器是基于溶液中DNA级联信号放大引起的纳米通道表面电荷的变化而设计的。该方法可间接定量检测目标miRNA（miR-122），检测限为97.2 aM，具有良好的选择性。所设计的纳米通道传感系统可以利用核酸的杂交和构型转换，结合级联的“或”逻辑门实现多种肝癌相关miRNA（miR-122和miR Let-7a）的智能检测，为肝癌的临床诊断和疗效评价提供理论指导和技术支持。
Nanochannel-based analytical techniques have great potential applications for nucleic acid sequencing and high sensitivity detection of biological molecules. However, the sensitivity of conventional solid-state nanochannel sensors is hampered by a lack of effective signal amplification strategies, which has limited its utility in the field of analytical chemistry. Here we selected a solid-state nanochannnel modified with polyethylenimine and Zr4+in combination with graphene oxide as the sensing platform. The high-performance sensor is based upon the change of the surface charge of the nanochannel, which is resulted from DNA cascade signal amplification in solution. The target miRNA (miR-122) can be indirectly quantitated with a detection limit of 97.2 aM with an excellent selectivity. Depending on the nucleic acid’s hybridization and configuration transform, the designed nanochannel sensing systems can realize the intelligent detection of multiple liver cancer-related miRNA (miR-122 and miR Let-7a) integrating with cascaded INHIBIT-OR logic gate to provide theoretical guidance and technical support for clinical diagnosis and therapeutic evaluation of liver cancer.